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Effects of tumor necrosis factor-α on cell proliferation, prostaglandins and matrix-metalloproteinases production in rat endometrial stromal cells cultured in vitro
Tumor necrosis factor‐α (TNF‐α) is known as a pluripotent cell mediator, and it is implicated in the control of uterine cell growth, differentiation and function during estrous cycle and pregnancy. In this study, we investigated the effect of TNF‐α on endometrial stromal cells derived from rat uteru...
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| Published in: | Journal of experimental zoology. Part A, Ecological genetics and physiology Ecological genetics and physiology, 2007-12, Vol.307A (12), p.699-707 |
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| Main Authors: | , , , , , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c3903-cd797de1934b4ca60f83cbda7b8cd76d8c6ecebb45ff53920d0df6273db10e713 |
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| cites | cdi_FETCH-LOGICAL-c3903-cd797de1934b4ca60f83cbda7b8cd76d8c6ecebb45ff53920d0df6273db10e713 |
| container_end_page | 707 |
| container_issue | 12 |
| container_start_page | 699 |
| container_title | Journal of experimental zoology. Part A, Ecological genetics and physiology |
| container_volume | 307A |
| creator | Gamo, Toru Yamauchi, Nobuhiko Nishimura, Kyohei Watanabe, Ryo Matsumoto, Kenji Oozono, Shinji Kubota, Kaiyu He, Pei-Jian Soh, Tomoki Hattori, Masa-aki |
| description | Tumor necrosis factor‐α (TNF‐α) is known as a pluripotent cell mediator, and it is implicated in the control of uterine cell growth, differentiation and function during estrous cycle and pregnancy. In this study, we investigated the effect of TNF‐α on endometrial stromal cells derived from rat uterus (rat endometrial stromal cells, RES). RES were isolated from rat endometrium at day 5 of pregnancy. Proliferation activities of RES were measured by using bromodeoxyuridine (BrdU) labeling kit, the productions of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) were measured by enzyme immunoassay kits and the production of matrix metalloproteinases (MMPs) was analyzed by gelatin‐zymography. TNF‐α, as well as epidermal growth factor and fibroblast growth factor‐2, significantly increased the proliferation activity of RES (P |
| doi_str_mv | 10.1002/jez.a.423 |
| format | article |
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In this study, we investigated the effect of TNF‐α on endometrial stromal cells derived from rat uterus (rat endometrial stromal cells, RES). RES were isolated from rat endometrium at day 5 of pregnancy. Proliferation activities of RES were measured by using bromodeoxyuridine (BrdU) labeling kit, the productions of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) were measured by enzyme immunoassay kits and the production of matrix metalloproteinases (MMPs) was analyzed by gelatin‐zymography. TNF‐α, as well as epidermal growth factor and fibroblast growth factor‐2, significantly increased the proliferation activity of RES (P<0.05). TNF‐α selectively stimulated the production of PGE2 in RES (P<0.05), but not the production of PGF2α. Additionally, TNF‐α did not stimulate the production of MMPs in RES at the concentration of 5 ng/mL, compared with the control groups (P>0.05). In conclusion, this study demonstrates several regulational functions of TNF‐α on RES using in vitro culture system. The effects of TNF‐α on proliferation and MMP production of RES have been shown for the first time. We believe that these results demonstrate part of the functions of TNF‐α in endometrium and contribute to the better understanding of endometrial functions. J. Exp. Zool. 307A:699–707, 2007. © 2007 Wiley‐Liss, Inc.</description><identifier>ISSN: 1932-5223</identifier><identifier>EISSN: 1932-5231</identifier><identifier>DOI: 10.1002/jez.a.423</identifier><identifier>PMID: 17963241</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Cell Proliferation - drug effects ; Cells, Cultured ; Dinoprost - metabolism ; Dinoprostone - metabolism ; Dose-Response Relationship, Drug ; Embryo, Mammalian - cytology ; Endometrium - drug effects ; Endometrium - metabolism ; Endometrium - pathology ; Epidermal Growth Factor - pharmacology ; Female ; Fibroblast Growth Factor 2 - pharmacology ; Male ; Matrix Metalloproteinases - metabolism ; Pregnancy ; Rats ; Rats, Wistar ; Stromal Cells - drug effects ; Stromal Cells - metabolism ; Stromal Cells - pathology ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Journal of experimental zoology. 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Part A, Ecological genetics and physiology</title><addtitle>J. Exp. Zool</addtitle><description>Tumor necrosis factor‐α (TNF‐α) is known as a pluripotent cell mediator, and it is implicated in the control of uterine cell growth, differentiation and function during estrous cycle and pregnancy. In this study, we investigated the effect of TNF‐α on endometrial stromal cells derived from rat uterus (rat endometrial stromal cells, RES). RES were isolated from rat endometrium at day 5 of pregnancy. Proliferation activities of RES were measured by using bromodeoxyuridine (BrdU) labeling kit, the productions of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) were measured by enzyme immunoassay kits and the production of matrix metalloproteinases (MMPs) was analyzed by gelatin‐zymography. TNF‐α, as well as epidermal growth factor and fibroblast growth factor‐2, significantly increased the proliferation activity of RES (P<0.05). TNF‐α selectively stimulated the production of PGE2 in RES (P<0.05), but not the production of PGF2α. Additionally, TNF‐α did not stimulate the production of MMPs in RES at the concentration of 5 ng/mL, compared with the control groups (P>0.05). In conclusion, this study demonstrates several regulational functions of TNF‐α on RES using in vitro culture system. The effects of TNF‐α on proliferation and MMP production of RES have been shown for the first time. We believe that these results demonstrate part of the functions of TNF‐α in endometrium and contribute to the better understanding of endometrial functions. J. Exp. Zool. 307A:699–707, 2007. © 2007 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Dinoprost - metabolism</subject><subject>Dinoprostone - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Embryo, Mammalian - cytology</subject><subject>Endometrium - drug effects</subject><subject>Endometrium - metabolism</subject><subject>Endometrium - pathology</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Female</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Male</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Stromal Cells - drug effects</subject><subject>Stromal Cells - metabolism</subject><subject>Stromal Cells - pathology</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>1932-5223</issn><issn>1932-5231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqF0c1qFTEUB_BBFFurC19AshIE55qvmcwspVyrUhWkKrgJmeREUjNJm2S09X18AF_EZzLDvdSVuDoJ-eWfj9M0DwneEIzps3P4sVEbTtmt5pCMjLYdZeT2zZiyg-ZezucYdxyP_G5zQMTYM8rJYfNzay3oklG0qCxzTCiATjG7jKzSJab29y8UA9LgPbpI0TsLSRUXw9N1mov64lUwLmRUC5pVSe6qnaEo72MFBVxQGfKKzaLXjcgFVCMQBBMrTE55lEuKc63rMRnpxZclgVnlN1eX7jd3rPIZHuzrUfPhxfbs-GV7-u7k1fHz01azEbNWGzEKA_XZfOJa9dgOTE9GiWmoS70ZdA8apol31nZspNhgY3sqmJkIBkHYUfN4l1tve7lALnJ2eb2TChCXLPuhwwPp-_9CWhkRGFf4ZAfXT80JrLxIblbpWhIs1-bJ2jypZG1etY_2ocs0g_kr992qoN2B787D9b-T5Ovt513g3rtc4OrGq_RV9oKJTn56eyIZF2d8fP9RvmF_AGRiuZI</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Gamo, Toru</creator><creator>Yamauchi, Nobuhiko</creator><creator>Nishimura, Kyohei</creator><creator>Watanabe, Ryo</creator><creator>Matsumoto, Kenji</creator><creator>Oozono, Shinji</creator><creator>Kubota, Kaiyu</creator><creator>He, Pei-Jian</creator><creator>Soh, Tomoki</creator><creator>Hattori, Masa-aki</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Effects of tumor necrosis factor-α on cell proliferation, prostaglandins and matrix-metalloproteinases production in rat endometrial stromal cells cultured in vitro</title><author>Gamo, Toru ; Yamauchi, Nobuhiko ; Nishimura, Kyohei ; Watanabe, Ryo ; Matsumoto, Kenji ; Oozono, Shinji ; Kubota, Kaiyu ; He, Pei-Jian ; Soh, Tomoki ; Hattori, Masa-aki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3903-cd797de1934b4ca60f83cbda7b8cd76d8c6ecebb45ff53920d0df6273db10e713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Dinoprost - metabolism</topic><topic>Dinoprostone - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Embryo, Mammalian - cytology</topic><topic>Endometrium - drug effects</topic><topic>Endometrium - metabolism</topic><topic>Endometrium - pathology</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Female</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Male</topic><topic>Matrix Metalloproteinases - metabolism</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Stromal Cells - drug effects</topic><topic>Stromal Cells - metabolism</topic><topic>Stromal Cells - pathology</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gamo, Toru</creatorcontrib><creatorcontrib>Yamauchi, Nobuhiko</creatorcontrib><creatorcontrib>Nishimura, Kyohei</creatorcontrib><creatorcontrib>Watanabe, Ryo</creatorcontrib><creatorcontrib>Matsumoto, Kenji</creatorcontrib><creatorcontrib>Oozono, Shinji</creatorcontrib><creatorcontrib>Kubota, Kaiyu</creatorcontrib><creatorcontrib>He, Pei-Jian</creatorcontrib><creatorcontrib>Soh, Tomoki</creatorcontrib><creatorcontrib>Hattori, Masa-aki</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental zoology. Part A, Ecological genetics and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gamo, Toru</au><au>Yamauchi, Nobuhiko</au><au>Nishimura, Kyohei</au><au>Watanabe, Ryo</au><au>Matsumoto, Kenji</au><au>Oozono, Shinji</au><au>Kubota, Kaiyu</au><au>He, Pei-Jian</au><au>Soh, Tomoki</au><au>Hattori, Masa-aki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of tumor necrosis factor-α on cell proliferation, prostaglandins and matrix-metalloproteinases production in rat endometrial stromal cells cultured in vitro</atitle><jtitle>Journal of experimental zoology. Part A, Ecological genetics and physiology</jtitle><addtitle>J. Exp. 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TNF‐α, as well as epidermal growth factor and fibroblast growth factor‐2, significantly increased the proliferation activity of RES (P<0.05). TNF‐α selectively stimulated the production of PGE2 in RES (P<0.05), but not the production of PGF2α. Additionally, TNF‐α did not stimulate the production of MMPs in RES at the concentration of 5 ng/mL, compared with the control groups (P>0.05). In conclusion, this study demonstrates several regulational functions of TNF‐α on RES using in vitro culture system. The effects of TNF‐α on proliferation and MMP production of RES have been shown for the first time. We believe that these results demonstrate part of the functions of TNF‐α in endometrium and contribute to the better understanding of endometrial functions. J. Exp. Zool. 307A:699–707, 2007. © 2007 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17963241</pmid><doi>10.1002/jez.a.423</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of experimental zoology. Part A, Ecological genetics and physiology, 2007-12, Vol.307A (12), p.699-707 |
| issn | 1932-5223 1932-5231 |
| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Animals Cell Proliferation - drug effects Cells, Cultured Dinoprost - metabolism Dinoprostone - metabolism Dose-Response Relationship, Drug Embryo, Mammalian - cytology Endometrium - drug effects Endometrium - metabolism Endometrium - pathology Epidermal Growth Factor - pharmacology Female Fibroblast Growth Factor 2 - pharmacology Male Matrix Metalloproteinases - metabolism Pregnancy Rats Rats, Wistar Stromal Cells - drug effects Stromal Cells - metabolism Stromal Cells - pathology Tumor Necrosis Factor-alpha - pharmacology |
| title | Effects of tumor necrosis factor-α on cell proliferation, prostaglandins and matrix-metalloproteinases production in rat endometrial stromal cells cultured in vitro |
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