Ligation of CD47 mediates phosphatidylserine expression on erythrocytes and a concomitant loss of viability in vitro

Summary CD47 ligation has been shown to induce phosphatidylserine (PS) expression as part of a death pathway in nucleated blood cells. Using Annexin V binding assays we showed that ligation of CD47 with the specific CD47‐binding peptide 41NK, anti‐CD47 monoclonal antibody, and its natural ligand thr...

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Bibliographic Details
Published in:British journal of haematology 2005-09, Vol.130 (5), p.788-790
Main Authors: Head, David J., Lee, Zoe E., Swallah, Muhammed M., Avent, Neil D.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - pharmacology
Antigen-Antibody Reactions
Antigens, CD - metabolism
Biological and medical sciences
CD47
CD47 Antigen
Cell Survival
Cells, Cultured
erythrocytes
Erythrocytes - metabolism
Erythrocytes - pathology
Hematologic and hematopoietic diseases
Hematology
Humans
Ligands
Medical sciences
phosphatidylserine
Phosphatidylserines - metabolism
Protein Binding
Thrombospondin 1 - metabolism
thrombospondin‐1
viability
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Summary:Summary CD47 ligation has been shown to induce phosphatidylserine (PS) expression as part of a death pathway in nucleated blood cells. Using Annexin V binding assays we showed that ligation of CD47 with the specific CD47‐binding peptide 41NK, anti‐CD47 monoclonal antibody, and its natural ligand thrombospondin‐1 also induced PS expression on enucleated erythrocytes. PS expression was associated with a concomitant loss of erythrocyte viability in vitro. Further characterisation of the CD47‐PS signalling pathway on erythrocytes may help develop clinical strategies to further preserve the life of blood donations and improve our understanding of certain types of haemolytic anaemias.
ISSN:0007-1048
1365-2141
DOI:10.1111/j.1365-2141.2005.05668.x