Platelet-rich plasma influence on human osteoblasts growth

Objective: The influence of progressively high concentrations of platelet‐rich plasma (PRP) on human osteoblast hFOB1.19 proliferation was evaluated. Material and methods: The PRP was obtained from a human source. Two experiments were conducted. In the first one, PRP was diluted to 50%, 25%, 12.5% a...

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Bibliographic Details
Published in:Clinical oral implants research 2005-08, Vol.16 (4), p.456-460
Main Authors: Ferreira, Cimara Fortes, Carriel Gomes, Márcia Cristina, Filho, José Scarso, Granjeiro, José Mauro, Oliveira Simões, Cláudia Maria, Magini, Ricardo de Souza
Format: Article
Language:English
Subjects:
Blood Platelets - physiology
Cell Division - physiology
Cell Proliferation
Cells, Cultured
Coloring Agents
Culture Media
Culture Media, Serum-Free
Dentistry
human osteoblasts
Humans
Male
Osteoblasts - physiology
Plasma
platelet-rich plasma
proliferation
Tetrazolium Salts
Thiazoles
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Summary:Objective: The influence of progressively high concentrations of platelet‐rich plasma (PRP) on human osteoblast hFOB1.19 proliferation was evaluated. Material and methods: The PRP was obtained from a human source. Two experiments were conducted. In the first one, PRP was diluted to 50%, 25%, 12.5% and 6.125% (v/v) with culture medium (Modified Eagle's Medium (MEM) : Ham's F12 Medium (HAM‐F12%) and 1% antibiotics–antimicotic) supplemented with 10% of fetal bovine serum (FBS). In the second experiment, all conditions were identical except for the absence of FBS in the culture medium. Results: The results of the osteoblast proliferation test were higher when stimulated by the 50% PRP dilution, with or without FBS. A further study is suggested to determine if concentrations above 50% could cause higher rates of osteoblast proliferation. In this study, the results were not statistically different (P
ISSN:0905-7161
1600-0501
DOI:10.1111/j.1600-0501.2005.01145.x