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Sexual Maturation Modulates Expression of Nuclear Receptor Types in Laser-Captured Single Cells of the Cichlid (Oreochromis niloticus) Pituitary
The role of steroid/thyroid hormones in the regulation of endocrine cells at the level of the pituitary has remained unclear. Therefore, using single-cell quantitative real-time PCR, we examined absolute amounts of transcripts for nuclear receptors [estrogen receptors (ERs) α, β, and γ; androgen rec...
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Published in: | Endocrinology (Philadelphia) 2007-12, Vol.148 (12), p.5822-5830 |
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creator | Kitahashi, Takashi Ogawa, Satoshi Soga, Tomoko Sakuma, Yasuo Parhar, Ishwar |
description | The role of steroid/thyroid hormones in the regulation of endocrine cells at the level of the pituitary has remained unclear. Therefore, using single-cell quantitative real-time PCR, we examined absolute amounts of transcripts for nuclear receptors [estrogen receptors (ERs) α, β, and γ; androgen receptors (ARs) a and b; glucocorticoid receptors (GRs) 1, 2a, and 2b; and thyroid hormone receptors (TRs) α1, α2, and β] in pituitary cells of immature (IM) and mature (M) male tilapia, Oreochromis niloticus. In the two reproductive stages, ACTH cells expressed only ERβ, whereas all other pituitary cell types expressed ERα + β, and a subpopulation coexpressed ARa, ARb, GR1, GR2b, and TRβ but lacked ERγ, GR2a, TRα1, and TRα2. IM males had high percentages of LH cells (IM 46.0% vs. M 10.0%), GH cells (IM 23.3% vs. M 7.9%), and prolactin cells (IM 68.8% vs. M 6.0%) with ERβ, and TSH cells (IM 19.2% vs. M 0.0%) and MSH cells (IM 25.6% vs. M 0.0%) with ERα + TRβ. A high percentage of FSH cells in IM males expressed ERβ (IM 46.9% vs. M 18.8%), and FSH cells in M males showed significantly high GR1 transcripts (IM 76.0 ± 5.0 vs. M 195.0 ± 10.7 copies per cell; P < 0.05), suggesting that FSH cells are regulated differently in the two reproductive stages. Coexpression of ERα + β in high percentages of cells of the GH family (GH, IM 43.8% vs. M 14.3%; prolactin, IM 8.3% vs. M 59.7%; somatolactin, IM 22.2% vs. M 42.2%) suggests that the expression of both ERs is important for functionality. Thus, differential coexpression of genes for nuclear receptors in subpopulations of pituitary cell types suggests multiple steroid/thyroid hormone regulatory pathways at the level of the pituitary during the two reproductive stages. |
doi_str_mv | 10.1210/en.2007-0311 |
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Therefore, using single-cell quantitative real-time PCR, we examined absolute amounts of transcripts for nuclear receptors [estrogen receptors (ERs) α, β, and γ; androgen receptors (ARs) a and b; glucocorticoid receptors (GRs) 1, 2a, and 2b; and thyroid hormone receptors (TRs) α1, α2, and β] in pituitary cells of immature (IM) and mature (M) male tilapia, Oreochromis niloticus. In the two reproductive stages, ACTH cells expressed only ERβ, whereas all other pituitary cell types expressed ERα + β, and a subpopulation coexpressed ARa, ARb, GR1, GR2b, and TRβ but lacked ERγ, GR2a, TRα1, and TRα2. IM males had high percentages of LH cells (IM 46.0% vs. M 10.0%), GH cells (IM 23.3% vs. M 7.9%), and prolactin cells (IM 68.8% vs. M 6.0%) with ERβ, and TSH cells (IM 19.2% vs. M 0.0%) and MSH cells (IM 25.6% vs. M 0.0%) with ERα + TRβ. A high percentage of FSH cells in IM males expressed ERβ (IM 46.9% vs. M 18.8%), and FSH cells in M males showed significantly high GR1 transcripts (IM 76.0 ± 5.0 vs. M 195.0 ± 10.7 copies per cell; P < 0.05), suggesting that FSH cells are regulated differently in the two reproductive stages. Coexpression of ERα + β in high percentages of cells of the GH family (GH, IM 43.8% vs. M 14.3%; prolactin, IM 8.3% vs. M 59.7%; somatolactin, IM 22.2% vs. M 42.2%) suggests that the expression of both ERs is important for functionality. Thus, differential coexpression of genes for nuclear receptors in subpopulations of pituitary cell types suggests multiple steroid/thyroid hormone regulatory pathways at the level of the pituitary during the two reproductive stages.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2007-0311</identifier><identifier>PMID: 17823257</identifier><identifier>CODEN: ENDOAO</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Adrenocorticotropic hormone ; Androgen receptors ; Animals ; Biological and medical sciences ; Cichlidae ; Cichlids - genetics ; Estrogen Receptor alpha - genetics ; Estrogen Receptor beta - genetics ; Estrogen Receptor beta - metabolism ; Estrogen receptors ; Estrogens ; Fish Proteins - genetics ; Fish Proteins - metabolism ; Follicle Stimulating Hormone - genetics ; Follicle Stimulating Hormone - metabolism ; Follicle-stimulating hormone ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Glucocorticoid receptors ; Glucocorticoids ; Glycoproteins - genetics ; Glycoproteins - metabolism ; Gonads - cytology ; Gonads - metabolism ; Growth Hormone - genetics ; Growth Hormone - metabolism ; Growth hormones ; Hormones ; Immunohistochemistry ; In Situ Hybridization ; Lasers ; Luteinizing hormone ; Luteinizing Hormone - genetics ; Luteinizing Hormone - metabolism ; Male ; Males ; Molecular Sequence Data ; Nuclear receptors ; Oreochromis niloticus ; Pituitary ; Pituitary Gland - cytology ; Pituitary Gland - metabolism ; Pituitary Hormones - genetics ; Pituitary Hormones - metabolism ; Prolactin ; Prolactin - genetics ; Prolactin - metabolism ; Real time ; Receptors ; Receptors, Androgen - genetics ; Receptors, Cytoplasmic and Nuclear - genetics ; Receptors, Estrogen - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sexual Maturation ; Steroids ; Subpopulations ; Thyroid ; Thyroid gland ; Thyroid hormone receptors ; Thyroid Hormone Receptors alpha - genetics ; Thyroid Hormone Receptors alpha - metabolism ; Thyroid Hormone Receptors beta - genetics ; Thyroid Hormone Receptors beta - metabolism ; Thyroid hormones ; Thyroid-stimulating hormone ; Thyrotropin - genetics ; Thyrotropin - metabolism ; Tilapia ; Vertebrates: endocrinology</subject><ispartof>Endocrinology (Philadelphia), 2007-12, Vol.148 (12), p.5822-5830</ispartof><rights>Copyright © 2007 by The Endocrine Society 2007</rights><rights>2008 INIST-CNRS</rights><rights>Copyright © 2007 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-e96c5815135db603bd492db96ccc7e059e8a899c3dea78b17a7d87212894ecac3</citedby><cites>FETCH-LOGICAL-c558t-e96c5815135db603bd492db96ccc7e059e8a899c3dea78b17a7d87212894ecac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19861853$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17823257$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitahashi, Takashi</creatorcontrib><creatorcontrib>Ogawa, Satoshi</creatorcontrib><creatorcontrib>Soga, Tomoko</creatorcontrib><creatorcontrib>Sakuma, Yasuo</creatorcontrib><creatorcontrib>Parhar, Ishwar</creatorcontrib><title>Sexual Maturation Modulates Expression of Nuclear Receptor Types in Laser-Captured Single Cells of the Cichlid (Oreochromis niloticus) Pituitary</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>The role of steroid/thyroid hormones in the regulation of endocrine cells at the level of the pituitary has remained unclear. Therefore, using single-cell quantitative real-time PCR, we examined absolute amounts of transcripts for nuclear receptors [estrogen receptors (ERs) α, β, and γ; androgen receptors (ARs) a and b; glucocorticoid receptors (GRs) 1, 2a, and 2b; and thyroid hormone receptors (TRs) α1, α2, and β] in pituitary cells of immature (IM) and mature (M) male tilapia, Oreochromis niloticus. In the two reproductive stages, ACTH cells expressed only ERβ, whereas all other pituitary cell types expressed ERα + β, and a subpopulation coexpressed ARa, ARb, GR1, GR2b, and TRβ but lacked ERγ, GR2a, TRα1, and TRα2. IM males had high percentages of LH cells (IM 46.0% vs. M 10.0%), GH cells (IM 23.3% vs. M 7.9%), and prolactin cells (IM 68.8% vs. M 6.0%) with ERβ, and TSH cells (IM 19.2% vs. M 0.0%) and MSH cells (IM 25.6% vs. M 0.0%) with ERα + TRβ. A high percentage of FSH cells in IM males expressed ERβ (IM 46.9% vs. M 18.8%), and FSH cells in M males showed significantly high GR1 transcripts (IM 76.0 ± 5.0 vs. M 195.0 ± 10.7 copies per cell; P < 0.05), suggesting that FSH cells are regulated differently in the two reproductive stages. Coexpression of ERα + β in high percentages of cells of the GH family (GH, IM 43.8% vs. M 14.3%; prolactin, IM 8.3% vs. M 59.7%; somatolactin, IM 22.2% vs. M 42.2%) suggests that the expression of both ERs is important for functionality. Thus, differential coexpression of genes for nuclear receptors in subpopulations of pituitary cell types suggests multiple steroid/thyroid hormone regulatory pathways at the level of the pituitary during the two reproductive stages.</description><subject>Adrenocorticotropic hormone</subject><subject>Androgen receptors</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cichlidae</subject><subject>Cichlids - genetics</subject><subject>Estrogen Receptor alpha - genetics</subject><subject>Estrogen Receptor beta - genetics</subject><subject>Estrogen Receptor beta - metabolism</subject><subject>Estrogen receptors</subject><subject>Estrogens</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>Follicle Stimulating Hormone - genetics</subject><subject>Follicle Stimulating Hormone - metabolism</subject><subject>Follicle-stimulating hormone</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Glucocorticoid receptors</subject><subject>Glucocorticoids</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - metabolism</subject><subject>Gonads - cytology</subject><subject>Gonads - metabolism</subject><subject>Growth Hormone - genetics</subject><subject>Growth Hormone - metabolism</subject><subject>Growth hormones</subject><subject>Hormones</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Lasers</subject><subject>Luteinizing hormone</subject><subject>Luteinizing Hormone - genetics</subject><subject>Luteinizing Hormone - metabolism</subject><subject>Male</subject><subject>Males</subject><subject>Molecular Sequence Data</subject><subject>Nuclear receptors</subject><subject>Oreochromis niloticus</subject><subject>Pituitary</subject><subject>Pituitary Gland - cytology</subject><subject>Pituitary Gland - metabolism</subject><subject>Pituitary Hormones - genetics</subject><subject>Pituitary Hormones - metabolism</subject><subject>Prolactin</subject><subject>Prolactin - genetics</subject><subject>Prolactin - metabolism</subject><subject>Real time</subject><subject>Receptors</subject><subject>Receptors, Androgen - genetics</subject><subject>Receptors, Cytoplasmic and Nuclear - genetics</subject><subject>Receptors, Estrogen - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sexual Maturation</subject><subject>Steroids</subject><subject>Subpopulations</subject><subject>Thyroid</subject><subject>Thyroid gland</subject><subject>Thyroid hormone receptors</subject><subject>Thyroid Hormone Receptors alpha - genetics</subject><subject>Thyroid Hormone Receptors alpha - metabolism</subject><subject>Thyroid Hormone Receptors beta - genetics</subject><subject>Thyroid Hormone Receptors beta - metabolism</subject><subject>Thyroid hormones</subject><subject>Thyroid-stimulating hormone</subject><subject>Thyrotropin - genetics</subject><subject>Thyrotropin - metabolism</subject><subject>Tilapia</subject><subject>Vertebrates: endocrinology</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkl1rFDEUhgdR7Fq981oColVwajKZTJJLWeoHbK3Yej1kM2fdlGwyzQe0_8Kf3Iw7sCCKV-EcnrznPbynqp4TfEoagt-DO20w5jWmhDyoFkS2rOaE44fVAmNCa940_Kh6EuN1Kdu2pY-rI8JFQxvGF9WvS7jNyqJzlXJQyXiHzv2QrUoQ0dntGCDGqek36GvWFlRA30HDmHxAV3djgYxDKxUh1Es1Fg0Y0KVxPy2gJVgbp49pWwqjt9YM6M1FAK-3we9MRM5Yn4zO8S36ZlI2SYW7p9WjjbIRns3vcfXj49nV8nO9uvj0ZflhVWvGRKpBdpoJwghlw7rDdD20shnWpas1B8wkCCWk1HQAxcWacMUHwRvSCNmCVpoeV6_3umPwNxli6osjXSwrBz7HvhNFm1P5X5BITmiLuwK-_AO89jm4skRPCcVMdFRO1Ls9pYOPMcCmH4PZlcV7gvsp0B5cPwXaT4EW_MUsmtc7GA7wnGABXs2AilrZTVBOm3jgpOiIYLRwJ3vO5_FfI-t5JN2T4Aavg3Hw-woO2_zV6D0z0cZ-</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Kitahashi, Takashi</creator><creator>Ogawa, Satoshi</creator><creator>Soga, Tomoko</creator><creator>Sakuma, Yasuo</creator><creator>Parhar, Ishwar</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7TK</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Sexual Maturation Modulates Expression of Nuclear Receptor Types in Laser-Captured Single Cells of the Cichlid (Oreochromis niloticus) Pituitary</title><author>Kitahashi, Takashi ; Ogawa, Satoshi ; Soga, Tomoko ; Sakuma, Yasuo ; Parhar, Ishwar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-e96c5815135db603bd492db96ccc7e059e8a899c3dea78b17a7d87212894ecac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adrenocorticotropic hormone</topic><topic>Androgen receptors</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cichlidae</topic><topic>Cichlids - genetics</topic><topic>Estrogen Receptor alpha - genetics</topic><topic>Estrogen Receptor beta - genetics</topic><topic>Estrogen Receptor beta - metabolism</topic><topic>Estrogen receptors</topic><topic>Estrogens</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>Follicle Stimulating Hormone - genetics</topic><topic>Follicle Stimulating Hormone - metabolism</topic><topic>Follicle-stimulating hormone</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Glucocorticoid receptors</topic><topic>Glucocorticoids</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - metabolism</topic><topic>Gonads - cytology</topic><topic>Gonads - metabolism</topic><topic>Growth Hormone - genetics</topic><topic>Growth Hormone - metabolism</topic><topic>Growth hormones</topic><topic>Hormones</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Lasers</topic><topic>Luteinizing hormone</topic><topic>Luteinizing Hormone - genetics</topic><topic>Luteinizing Hormone - metabolism</topic><topic>Male</topic><topic>Males</topic><topic>Molecular Sequence Data</topic><topic>Nuclear receptors</topic><topic>Oreochromis niloticus</topic><topic>Pituitary</topic><topic>Pituitary Gland - cytology</topic><topic>Pituitary Gland - metabolism</topic><topic>Pituitary Hormones - genetics</topic><topic>Pituitary Hormones - metabolism</topic><topic>Prolactin</topic><topic>Prolactin - genetics</topic><topic>Prolactin - metabolism</topic><topic>Real time</topic><topic>Receptors</topic><topic>Receptors, Androgen - genetics</topic><topic>Receptors, Cytoplasmic and Nuclear - genetics</topic><topic>Receptors, Estrogen - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sexual Maturation</topic><topic>Steroids</topic><topic>Subpopulations</topic><topic>Thyroid</topic><topic>Thyroid gland</topic><topic>Thyroid hormone receptors</topic><topic>Thyroid Hormone Receptors alpha - genetics</topic><topic>Thyroid Hormone Receptors alpha - metabolism</topic><topic>Thyroid Hormone Receptors beta - genetics</topic><topic>Thyroid Hormone Receptors beta - metabolism</topic><topic>Thyroid hormones</topic><topic>Thyroid-stimulating hormone</topic><topic>Thyrotropin - genetics</topic><topic>Thyrotropin - metabolism</topic><topic>Tilapia</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitahashi, Takashi</creatorcontrib><creatorcontrib>Ogawa, Satoshi</creatorcontrib><creatorcontrib>Soga, Tomoko</creatorcontrib><creatorcontrib>Sakuma, Yasuo</creatorcontrib><creatorcontrib>Parhar, Ishwar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitahashi, Takashi</au><au>Ogawa, Satoshi</au><au>Soga, Tomoko</au><au>Sakuma, Yasuo</au><au>Parhar, Ishwar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sexual Maturation Modulates Expression of Nuclear Receptor Types in Laser-Captured Single Cells of the Cichlid (Oreochromis niloticus) Pituitary</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>148</volume><issue>12</issue><spage>5822</spage><epage>5830</epage><pages>5822-5830</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><coden>ENDOAO</coden><abstract>The role of steroid/thyroid hormones in the regulation of endocrine cells at the level of the pituitary has remained unclear. Therefore, using single-cell quantitative real-time PCR, we examined absolute amounts of transcripts for nuclear receptors [estrogen receptors (ERs) α, β, and γ; androgen receptors (ARs) a and b; glucocorticoid receptors (GRs) 1, 2a, and 2b; and thyroid hormone receptors (TRs) α1, α2, and β] in pituitary cells of immature (IM) and mature (M) male tilapia, Oreochromis niloticus. In the two reproductive stages, ACTH cells expressed only ERβ, whereas all other pituitary cell types expressed ERα + β, and a subpopulation coexpressed ARa, ARb, GR1, GR2b, and TRβ but lacked ERγ, GR2a, TRα1, and TRα2. IM males had high percentages of LH cells (IM 46.0% vs. M 10.0%), GH cells (IM 23.3% vs. M 7.9%), and prolactin cells (IM 68.8% vs. M 6.0%) with ERβ, and TSH cells (IM 19.2% vs. M 0.0%) and MSH cells (IM 25.6% vs. M 0.0%) with ERα + TRβ. A high percentage of FSH cells in IM males expressed ERβ (IM 46.9% vs. M 18.8%), and FSH cells in M males showed significantly high GR1 transcripts (IM 76.0 ± 5.0 vs. M 195.0 ± 10.7 copies per cell; P < 0.05), suggesting that FSH cells are regulated differently in the two reproductive stages. Coexpression of ERα + β in high percentages of cells of the GH family (GH, IM 43.8% vs. M 14.3%; prolactin, IM 8.3% vs. M 59.7%; somatolactin, IM 22.2% vs. M 42.2%) suggests that the expression of both ERs is important for functionality. Thus, differential coexpression of genes for nuclear receptors in subpopulations of pituitary cell types suggests multiple steroid/thyroid hormone regulatory pathways at the level of the pituitary during the two reproductive stages.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>17823257</pmid><doi>10.1210/en.2007-0311</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adrenocorticotropic hormone Androgen receptors Animals Biological and medical sciences Cichlidae Cichlids - genetics Estrogen Receptor alpha - genetics Estrogen Receptor beta - genetics Estrogen Receptor beta - metabolism Estrogen receptors Estrogens Fish Proteins - genetics Fish Proteins - metabolism Follicle Stimulating Hormone - genetics Follicle Stimulating Hormone - metabolism Follicle-stimulating hormone Freshwater Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Glucocorticoid receptors Glucocorticoids Glycoproteins - genetics Glycoproteins - metabolism Gonads - cytology Gonads - metabolism Growth Hormone - genetics Growth Hormone - metabolism Growth hormones Hormones Immunohistochemistry In Situ Hybridization Lasers Luteinizing hormone Luteinizing Hormone - genetics Luteinizing Hormone - metabolism Male Males Molecular Sequence Data Nuclear receptors Oreochromis niloticus Pituitary Pituitary Gland - cytology Pituitary Gland - metabolism Pituitary Hormones - genetics Pituitary Hormones - metabolism Prolactin Prolactin - genetics Prolactin - metabolism Real time Receptors Receptors, Androgen - genetics Receptors, Cytoplasmic and Nuclear - genetics Receptors, Estrogen - genetics Reverse Transcriptase Polymerase Chain Reaction Sexual Maturation Steroids Subpopulations Thyroid Thyroid gland Thyroid hormone receptors Thyroid Hormone Receptors alpha - genetics Thyroid Hormone Receptors alpha - metabolism Thyroid Hormone Receptors beta - genetics Thyroid Hormone Receptors beta - metabolism Thyroid hormones Thyroid-stimulating hormone Thyrotropin - genetics Thyrotropin - metabolism Tilapia Vertebrates: endocrinology |
title | Sexual Maturation Modulates Expression of Nuclear Receptor Types in Laser-Captured Single Cells of the Cichlid (Oreochromis niloticus) Pituitary |
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