Influence of particle size, antigen load, dose and additional adjuvant on the immune response from antigen loaded PLA microparticles

Polylactide (PLA) polymer particles entrapping tetanus toxoid (TT) were evaluated in terms of particle size, antigen load, dose and additional adjuvant for achieving high and sustained anti-TT antibody titer from single point intramuscular immunization. Admixture of polymer entrapped TT and alum imp...

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Bibliographic Details
Published in:International journal of pharmaceutics 2005-09, Vol.301 (1), p.149-160
Main Authors: Katare, Yogesh K., Muthukumaran, T., Panda, Amulya K.
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - chemistry
Alum Compounds
Animals
Antibody response
Antigen load
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay
Female
General pharmacology
Immunization
Immunoglobulin G - analysis
Immunoglobulin G - biosynthesis
Injections, Intramuscular
Medical sciences
Microscopy, Electron, Scanning
Microspheres
Particle Size
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
PLA particles
Polyesters - chemistry
Rats
Rats, Wistar
Surface Properties
Tetanus toxoid
Tetanus Toxoid - administration & dosage
Tetanus Toxoid - chemistry
Tetanus Toxoid - immunology
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Summary:Polylactide (PLA) polymer particles entrapping tetanus toxoid (TT) were evaluated in terms of particle size, antigen load, dose and additional adjuvant for achieving high and sustained anti-TT antibody titer from single point intramuscular immunization. Admixture of polymer entrapped TT and alum improved the immune response in comparison to particle-based immunization. High and long lasting antibody titer was achieved upon immunization with 2–8 μm size particles. Microparticles within the size range 50–150 μm elicited very low serum antibody response. Immunization with very small particles (
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2005.05.028