Effects of protein-protein interaction in ultrafiltration based fractionation processes

This paper discusses the use of pulsed sample injection ultrafiltration (UF) for investigating protein–protein interaction, particularly its effect on protein transmission through UF membranes. Several binary protein mixtures were investigated; the proteins in each mixture being selected such that o...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2005-09, Vol.91 (6), p.678-687
Main Authors: Filipe, Carlos DM, Ghosh, Raja
Format: Article
Language:English
Subjects:
Associative
Biological and medical sciences
bioseparation
Biotechnology
Chemical Fractionation - methods
Chemical reactions
Correlation
Fractionation
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Membranes
Methods. Procedures. Technologies
Muramidase - chemistry
Muramidase - metabolism
Myoglobin - chemistry
Myoglobin - metabolism
Oligopeptides - chemistry
Oligopeptides - metabolism
Others
protein
Protein Interaction Mapping - methods
protein-protein interaction
Proteins
Proteins - chemistry
Proteins - metabolism
Serum Albumin, Bovine - chemistry
Serum Albumin, Bovine - metabolism
transmission
Ultrafiltration
Ultrafiltration - methods
Various methods and equipments
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Summary:This paper discusses the use of pulsed sample injection ultrafiltration (UF) for investigating protein–protein interaction, particularly its effect on protein transmission through UF membranes. Several binary protein mixtures were investigated; the proteins in each mixture being selected such that one of the proteins in the pair would be preferentially transmitted while the other would be either totally or substantially retained. The “retained” protein either decreased or increased or did not affect the sieving coefficient of the “transmitted” protein, this depending the type of protein–protein interaction, that is, associative, repulsive, or neutral. The type of protein–protein interaction depended on the particular protein pair under investigation as well as on the operating conditions used (pH and salt concentration). The magnitude of either decrease or increase in transmission of a preferentially transmitted protein due to the presence of a retained protein was found to be independent of the manner in which the proteins were injected into the system, that is, simultaneous or sequential. These magnitudes however correlated well with the ratio of the two proteins present in the feed. © 2005 Wiley Periodicals, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.20568