High-throughput analysis of Vitamin C in human plasma with the use of HPLC with monolithic column and UV-detection

Vitamin C plays a central role in the body. One of its important functions is its role as an antioxidant, and accurate measurements are important for interpretations of this role. However, its reactive nature and instability complicates the assessment, especially in biological samples. A high-throug...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-09, Vol.824 (1), p.132-138
Main Authors: Karlsen, Anette, Blomhoff, Rune, Gundersen, Thomas E.
Format: Article
Language:English
Subjects:
Adult
Analysis
Analytical, structural and metabolic biochemistry
Ascorbic acid
Ascorbic Acid - blood
Ascorbic Acid - standards
Biological and medical sciences
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Dehydroascorbic acid
Dehydroascorbic Acid - blood
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Infant
Medical sciences
Middle Aged
Monolithic column
Pharmacology. Drug treatments
Plasma
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet - methods
Vitamin C
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Summary:Vitamin C plays a central role in the body. One of its important functions is its role as an antioxidant, and accurate measurements are important for interpretations of this role. However, its reactive nature and instability complicates the assessment, especially in biological samples. A high-throughput chromatographic method using monolithic column and UV-detection was developed for the assessment of plasma ascorbic acid and total ascorbic acid. The method showed excellent analytical sensitivity, specificity, precision, recovery and linearity during the validation study. The method was used for the assessment of ascorbic acid and total ascorbic acid during several clinical studies.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2005.07.008