A new and simple solid-phase extraction method for LC determination of pyronaridine in human plasma

A new approach using a simple solid-phase extraction technique has been developed for the determination of pyronaridine (PND), an antimalarial drug, in human plasma. After extraction with C 18 solid-phase sorbent, PND was analyzed using a reverse phase chromatographic method with fluorescence detect...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-09, Vol.824 (1), p.45-50
Main Authors: Ramanathan, S., Karupiah, S., Nair, N.K., Olliaro, P.L., Navaratnam, V., Wernsdorfer, W.H., Mansor, S.M.
Format: Article
Language:English
Subjects:
Administration, Oral
Analysis
Analytical, structural and metabolic biochemistry
Antimalarials - blood
Antimalarials - isolation & purification
Antimalarials - pharmacokinetics
Area Under Curve
Biological and medical sciences
Calibration
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Half-Life
Humans
Malaria
Malaria, Falciparum - blood
Malaria, Falciparum - drug therapy
Medical sciences
Naphthyridines - blood
Naphthyridines - isolation & purification
Naphthyridines - pharmacokinetics
Pharmacology. Drug treatments
Plasma
Pyronaridine
Regression Analysis
Reproducibility of Results
Time Factors
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Description
Summary:A new approach using a simple solid-phase extraction technique has been developed for the determination of pyronaridine (PND), an antimalarial drug, in human plasma. After extraction with C 18 solid-phase sorbent, PND was analyzed using a reverse phase chromatographic method with fluorescence detection (at λ ex = 267 nm and λ em = 443 nm). The mean extraction recovery for PND was 95.2%. The coefficient of variation for intra-assay precision, inter-assay precision and accuracy was less than 10%. The quantification limit with fluorescence detection was 0.010 μg/mL plasma. The method described herein has several advantages over other published methods since it is easy to perform and rapid. It also permits reducing both, solvent use and sample preparation time. The method has been used successfully to assay plasma samples from clinical pharmacokinetic studies.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2005.06.034