A chimeric SM5-1 antibody inhibits hepatocellular carcinoma cell growth and induces caspase-dependent apoptosis

• Published in: Cancer letters 2007-12, Vol.258 (2), p.208-214
• Main Authors: Dai, Jianxin, Jin, Jun, Li, Bohua, Wang, Hao, Hou, Sheng, Qian, Weizhu, Kou, Geng, Zhang, Dapeng, Li, Jing, Tan, Min, Ma, Jing, Guo, Yajun
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - metabolism; Antibodies, Monoclonal - pharmacology; Apoptosis; Apoptosis - drug effects; Blotting, Western; Breast cancer; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Carrier Proteins - metabolism; Caspase 10 - metabolism; Caspase Inhibitors; Caspase-10; Caspases - metabolism; Cell growth; Cell Line, Tumor; Cell Proliferation - drug effects; Chimeric antibody; Cysteine Proteinase Inhibitors - pharmacology; Deoxyribonucleic acid; DNA; Dose-Response Relationship, Drug; Flow cytometry; Hematology, Oncology and Palliative Medicine; Hepatocellular carcinoma; Humans; Immunoprecipitation; Liver cancer; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Melanoma; Mice; Proteins; Science; SM5-1
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2007.09.002

Abstract cSM5-1 is a mouse-human chimeric antibody which has a high specificity for hepatocellular carcinoma, melanoma and breast cancer. In this study, cSM5-1 was found to be able to inhibit cell growth and induce apoptosis in hepatocellular carcinoma cells. The antitumor activity of cSM5-1 was closely correlated with the expression level of the SM5-1 binding protein in the cancer cells. The role of caspases in cSM5-1-induced apoptosis was also investigated, indicating that cSM5-1-induced apoptosis was partially caspase-dependent and caspase-10 played a critical role. These in vitro data indicate that cSM5-1 has the potential to be a promising candidate for cancer treatment.