Sequential injection extraction based on restricted access material for determination of furosemide in serum

Restricted access material (RAM) column containing 25 μm C 18 alkyl-diol support was integrated into the sequential injection analysis (SIA) manifold and the SIA–RAM system was tested for direct determination of furosemide in serum. LiChrospher ® ADS column based on restricted access material is pro...

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Bibliographic Details
Published in:Journal of Chromatography A 2005-09, Vol.1087 (1), p.245-251
Main Authors: Huclová, Jitka, Šatínský, Dalibor, Maia, Tiago, Karlíček, Rolf, Solich, Petr, Araújo, Alberto Nova
Format: Article
Language:English
Subjects:
Adsorption
Antihypertensive agents
Biological and medical sciences
Calibration
Cardiovascular system
Chromatography, High Pressure Liquid - methods
Diuretics - blood
Furosemide
Furosemide - blood
Hydrogen-Ion Concentration
Medical sciences
Pharmacology. Drug treatments
Reference Standards
Reproducibility of Results
Restricted access materials
Sensitivity and Specificity
Sequential injection analysis
Serum
Spectrometry, Fluorescence
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Summary:Restricted access material (RAM) column containing 25 μm C 18 alkyl-diol support was integrated into the sequential injection analysis (SIA) manifold and the SIA–RAM system was tested for direct determination of furosemide in serum. LiChrospher ® ADS column based on restricted access material is proposed to direct injection of biofluids. The integration of RAM material into SIA enabled creation of a comprehensive on-line sample clean-up technique combined with fluorescence quantitation of analyte. Centrifuged and diluted serum sample was aspirated into the system and loaded onto the column using acetonitrile–water (2:98), pH 2.7. The analyte was retained on the column while proteins contained in the sample were removed to the waste without precipitation and clogging the column. Interfering substances complicating the detection were washed out by acetonitrile–water (15:85), pH 2.7 in the next step. The extracted analyte was eluted by means of acetonitrile–water (25:75), pH 2.3 to the fluorescence detector (emission filter 385 nm). The whole procedure comprising sample pre-treatment, analyte detection and column reconditioning took 20 min. The recoveries of furosemide from serum lay between 101.4 and 103.4% for three concentrations of analyte.
ISSN:0021-9673
DOI:10.1016/j.chroma.2004.11.055