Regular arrangement of periodates bound to lysozyme

The structure of tetragonal hen egg‐white lysozyme soaked in a periodate solution has been determined to a resolution of 1.8 Å. Four high‐occupancy periodate positions have been identified on the basis of the anomalous signal of the I atoms. The four periodates exhibit a regular rectangular arrangem...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2005-09, Vol.61 (9), p.1181-1189
Main Authors: Ondráček, Jan, Weiss, Manfred S., Brynda, Jiří, Fiala, Jaroslav, Jursík, František, Řezáčová, Pavlína, Jenner, Lasse B., Sedláček, Juraj
Format: Article
Language:English
Subjects:
Animals
Chickens
crystal soaking
Crystallography, X-Ray
Egg Proteins
epitaxy
Female
hen egg-white lysozyme
Hydrogen Bonding
Molecular Structure
Muramidase - chemistry
periodate
Periodic Acid - chemistry
Protein Conformation
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Summary:The structure of tetragonal hen egg‐white lysozyme soaked in a periodate solution has been determined to a resolution of 1.8 Å. Four high‐occupancy periodate positions have been identified on the basis of the anomalous signal of the I atoms. The four periodates exhibit a regular rectangular arrangement on the surface of the lysozyme molecule. No similar regular arrangement was found either in lysozyme crystals soaked in other heavy‐atom anions or in other structures from the Protein Data Bank. Depending on their position on the surface of the protein, the periodate ions deviate to a varying extent from ideal octahedral geometry.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S090744490501543X