Monoxenic production of the entomopathogenic nematode Steinernema carpocapsae using culture media containing agave juice (aguamiel) from Mexican maguey-pulquero (Agave spp). Effects of the contents of nitrogen, carbohydrates and fat on infective juvenile production

The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2005-07, Vol.68 (1), p.91-97
Main Authors: ISLAS-LOPEZ, Marco-Antonio, SANJUAN-GALINDO, René, RODRIGUEZ-HERNANDEZ, Adriana-Inés, CHAVARRIA-HERNANDEZ, Norberto
Format: Article
Language:English
Subjects:
Agave
Agave - chemistry
Animals
Bacteria
Biological and medical sciences
Biotechnology
Carbohydrate Metabolism
Carbohydrates
Culture media
Culture Media - chemistry
Fats - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Mexico
Nematoda
Nematodes
Nitrogen
Nitrogen - metabolism
Pest Control, Biological
Rhabditida - growth & development
Rhabditida - metabolism
Rhabditida - microbiology
Steinernema carpocapsae
Time Factors
Xenorhabdus
Xenorhabdus nematophilus
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Summary:The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (aguamiel) from Mexican maguey-pulquero (Agave spp) as the main carbohydrate source. After 20 days of fermentation, a maximum viable IJ concentration of 249,000 IJ/ml and an initial nematode population multiplication factor of x620 were achieved when medium M4 was used (aguamiel concentration in this medium was 28% by vol.). M4 medium contained (w/v): 0.3% total nitrogen, 3.2% total carbohydrates and 3.0% total fat. According to the results obtained, total carbohydrates concentration appeared to be of great importance in obtaining high IJ concentrations.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-004-1818-9