Characterization of Surface-Modified Nanoparticles for in Vivo Biointeraction. A Sedimentation Field Flow Fractionation Study

Sedimentation field flow fractionation (SdFFF) is an emerging high-performance analytical tool for separation and determination of size and adsorption characteristics of colloidal particles. This study demonstrates how SdFFF can be used to characterize nanoparticles prepared for in vivo applications...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2005-09, Vol.77 (17), p.5488-5493
Main Authors: Andersson, Margaretha, Fromell, Karin, Gullberg, Elisabet, Artursson, Per, Caldwell, Karin D
Format: Article
Language:English
Subjects:
Analytical chemistry
Biology
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Fractionation
Fractionation, Field Flow - methods
Ligands
Microscopy, Electron, Scanning
Models, Biological
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Oligopeptides - analysis
Oligopeptides - metabolism
Other chromatographic methods
Polymers
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Summary:Sedimentation field flow fractionation (SdFFF) is an emerging high-performance analytical tool for separation and determination of size and adsorption characteristics of colloidal particles. This study demonstrates how SdFFF can be used to characterize nanoparticles prepared for in vivo applications including (1) the quantification of polymer uptake on nanoparticles where surface coverage is crucial and (2) the coupling of cell adhesive peptides containing the Arg-Gly-Asp motif (RGD). Quantitative information about polymer adhesion in order to prepare a bioinert surface and an accurate determination of ligand uptake are both of obvious importance for the understanding of, for example, relations between the number of attached molecules for biointeraction and an observed therapeutic effect. In addition, the present work highlights the necessity to perform careful characterization of commercially available particulate starting materials, in terms of size and polydispersity, prior to biological experimentation.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac050631h