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methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes
Seven isoforms of 85 kDa polypeptides (p85) were identified as methionine synthase (MetE) homologs by partial aminoacid sequencing in tobacco pollen tube extracts. Immunocytochemistry data showed a localization of the antigen on the surface of tip-focussed post-Golgi secretory vesicles (SVs), that a...
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| Published in: | Planta 2005-08, Vol.221 (6), p.776-789 |
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| Main Authors: | , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c402t-3b00f96e250acdaedc3a40a28ef6264730501cb1982c1d34005d22a6ffea0d6d3 |
| container_end_page | 789 |
| container_issue | 6 |
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| container_title | Planta |
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| creator | Moscatelli, A Scali, M Prescianotto-Baschong, C Ferro, M Garin, J Vignani, R Ciampolini, F Cresti, M |
| description | Seven isoforms of 85 kDa polypeptides (p85) were identified as methionine synthase (MetE) homologs by partial aminoacid sequencing in tobacco pollen tube extracts. Immunocytochemistry data showed a localization of the antigen on the surface of tip-focussed post-Golgi secretory vesicles (SVs), that appear to be partially associated with microtubules (Mts). The chemical dissection of pollen tube high speed supernatant (HSS) showed that two distinct pools of MetE are present in pollen tubes, one being the more acidic isoforms sedimenting at 15S and the remaining at 4S after zonal centrifugation through a sucrose density gradient. The identification of the MetE within the pollen tube and its possible participation as methyl donor in a wide range of metabolic reactions, makes it a good subject for studies on pollen tube growth regulation. |
| doi_str_mv | 10.1007/s00425-005-1487-7 |
| format | article |
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Immunocytochemistry data showed a localization of the antigen on the surface of tip-focussed post-Golgi secretory vesicles (SVs), that appear to be partially associated with microtubules (Mts). The chemical dissection of pollen tube high speed supernatant (HSS) showed that two distinct pools of MetE are present in pollen tubes, one being the more acidic isoforms sedimenting at 15S and the remaining at 4S after zonal centrifugation through a sucrose density gradient. The identification of the MetE within the pollen tube and its possible participation as methyl donor in a wide range of metabolic reactions, makes it a good subject for studies on pollen tube growth regulation.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-005-1487-7</identifier><identifier>PMID: 15940464</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase - metabolism ; Agronomy. Soil science and plant productions ; Amino Acid Sequence ; amino acid sequences ; Antibodies ; Antiserum ; Biological and medical sciences ; Centrifugation ; Cytoplasm ; Economic plant physiology ; Flowering, floral biology, reproduction patterns ; Fluorescent antibody techniques ; Fundamental and applied biological sciences. Psychology ; Gels ; Growth and development ; Isoenzymes ; methionine ; methionine synthase ; methyltransferases ; microtubules ; Molecular Sequence Data ; Nicotiana - enzymology ; Nicotiana - ultrastructure ; Nicotiana tabacum ; Organelles ; plant biochemistry ; Plant cells ; Plant Proteins ; Pollen ; Pollen - enzymology ; Pollen - ultrastructure ; Pollen tubes ; polypeptides ; Protein isoforms ; Secretory Vesicles - enzymology ; Secretory Vesicles - ultrastructure ; sequence alignment ; Sequence Homology, Amino Acid ; tobacco ; Western blotting</subject><ispartof>Planta, 2005-08, Vol.221 (6), p.776-789</ispartof><rights>Springer-Verlag Berlin Heidelberg 2005</rights><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-3b00f96e250acdaedc3a40a28ef6264730501cb1982c1d34005d22a6ffea0d6d3</citedby><cites>FETCH-LOGICAL-c402t-3b00f96e250acdaedc3a40a28ef6264730501cb1982c1d34005d22a6ffea0d6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23388990$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23388990$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17062506$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15940464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moscatelli, A</creatorcontrib><creatorcontrib>Scali, M</creatorcontrib><creatorcontrib>Prescianotto-Baschong, C</creatorcontrib><creatorcontrib>Ferro, M</creatorcontrib><creatorcontrib>Garin, J</creatorcontrib><creatorcontrib>Vignani, R</creatorcontrib><creatorcontrib>Ciampolini, F</creatorcontrib><creatorcontrib>Cresti, M</creatorcontrib><title>methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes</title><title>Planta</title><addtitle>Planta</addtitle><description>Seven isoforms of 85 kDa polypeptides (p85) were identified as methionine synthase (MetE) homologs by partial aminoacid sequencing in tobacco pollen tube extracts. Immunocytochemistry data showed a localization of the antigen on the surface of tip-focussed post-Golgi secretory vesicles (SVs), that appear to be partially associated with microtubules (Mts). The chemical dissection of pollen tube high speed supernatant (HSS) showed that two distinct pools of MetE are present in pollen tubes, one being the more acidic isoforms sedimenting at 15S and the remaining at 4S after zonal centrifugation through a sucrose density gradient. The identification of the MetE within the pollen tube and its possible participation as methyl donor in a wide range of metabolic reactions, makes it a good subject for studies on pollen tube growth regulation.</description><subject>5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase - metabolism</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Antibodies</subject><subject>Antiserum</subject><subject>Biological and medical sciences</subject><subject>Centrifugation</subject><subject>Cytoplasm</subject><subject>Economic plant physiology</subject><subject>Flowering, floral biology, reproduction patterns</subject><subject>Fluorescent antibody techniques</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Growth and development</subject><subject>Isoenzymes</subject><subject>methionine</subject><subject>methionine synthase</subject><subject>methyltransferases</subject><subject>microtubules</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - enzymology</subject><subject>Nicotiana - ultrastructure</subject><subject>Nicotiana tabacum</subject><subject>Organelles</subject><subject>plant biochemistry</subject><subject>Plant cells</subject><subject>Plant Proteins</subject><subject>Pollen</subject><subject>Pollen - enzymology</subject><subject>Pollen - ultrastructure</subject><subject>Pollen tubes</subject><subject>polypeptides</subject><subject>Protein isoforms</subject><subject>Secretory Vesicles - enzymology</subject><subject>Secretory Vesicles - ultrastructure</subject><subject>sequence alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>tobacco</subject><subject>Western blotting</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpdkE2P1DAMhiMEYoeFH8ABiJDgVnA-mqZHtOJLWokD7IFTlKbuTkZtM8QtaP49WXXESpxs6338yn4Zey7gnQBo3hOAlnUFUFdC26ZqHrCd0EpWErR9yHYApYdW1RfsCdEBoIhN85hdiLrVoI3esZ8TLvuY5jgjp9O87D0h36cpjemWR-KeKIXoF-z5n7jsOWHIuKR84r-RYhiReJz5kjofQuLHNI5YxrVDesoeDX4kfHaul-zm08cfV1-q62-fv159uK6CBrlUqgMYWoOyBh96j31QXoOXFgcjjW4U1CBCJ1org-iVLs_2UnozDOihN726ZG8332NOv1akxU2RAo6jnzGt5IytVSOkLODr_8BDWvNcbnNWWGskKFEgsUEhJ6KMgzvmOPl8cgLcXehuC92VO9xd6K4pOy_Pxms3YX-_cU65AG_OgKfgxyH7OUS65xow5X1TuBcbd6AS8T9dKmVt20LRX2364JPzt7l43HyXIBQIMAYK8ReKU5z4</recordid><startdate>20050801</startdate><enddate>20050801</enddate><creator>Moscatelli, A</creator><creator>Scali, M</creator><creator>Prescianotto-Baschong, C</creator><creator>Ferro, M</creator><creator>Garin, J</creator><creator>Vignani, R</creator><creator>Ciampolini, F</creator><creator>Cresti, M</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050801</creationdate><title>methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes</title><author>Moscatelli, A ; Scali, M ; Prescianotto-Baschong, C ; Ferro, M ; Garin, J ; Vignani, R ; Ciampolini, F ; Cresti, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-3b00f96e250acdaedc3a40a28ef6264730501cb1982c1d34005d22a6ffea0d6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase - metabolism</topic><topic>Agronomy. 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Psychology</topic><topic>Gels</topic><topic>Growth and development</topic><topic>Isoenzymes</topic><topic>methionine</topic><topic>methionine synthase</topic><topic>methyltransferases</topic><topic>microtubules</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - enzymology</topic><topic>Nicotiana - ultrastructure</topic><topic>Nicotiana tabacum</topic><topic>Organelles</topic><topic>plant biochemistry</topic><topic>Plant cells</topic><topic>Plant Proteins</topic><topic>Pollen</topic><topic>Pollen - enzymology</topic><topic>Pollen - ultrastructure</topic><topic>Pollen tubes</topic><topic>polypeptides</topic><topic>Protein isoforms</topic><topic>Secretory Vesicles - enzymology</topic><topic>Secretory Vesicles - ultrastructure</topic><topic>sequence alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>tobacco</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moscatelli, A</creatorcontrib><creatorcontrib>Scali, M</creatorcontrib><creatorcontrib>Prescianotto-Baschong, C</creatorcontrib><creatorcontrib>Ferro, M</creatorcontrib><creatorcontrib>Garin, J</creatorcontrib><creatorcontrib>Vignani, R</creatorcontrib><creatorcontrib>Ciampolini, F</creatorcontrib><creatorcontrib>Cresti, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moscatelli, A</au><au>Scali, M</au><au>Prescianotto-Baschong, C</au><au>Ferro, M</au><au>Garin, J</au><au>Vignani, R</au><au>Ciampolini, F</au><au>Cresti, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>2005-08-01</date><risdate>2005</risdate><volume>221</volume><issue>6</issue><spage>776</spage><epage>789</epage><pages>776-789</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Seven isoforms of 85 kDa polypeptides (p85) were identified as methionine synthase (MetE) homologs by partial aminoacid sequencing in tobacco pollen tube extracts. Immunocytochemistry data showed a localization of the antigen on the surface of tip-focussed post-Golgi secretory vesicles (SVs), that appear to be partially associated with microtubules (Mts). The chemical dissection of pollen tube high speed supernatant (HSS) showed that two distinct pools of MetE are present in pollen tubes, one being the more acidic isoforms sedimenting at 15S and the remaining at 4S after zonal centrifugation through a sucrose density gradient. The identification of the MetE within the pollen tube and its possible participation as methyl donor in a wide range of metabolic reactions, makes it a good subject for studies on pollen tube growth regulation.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>15940464</pmid><doi>10.1007/s00425-005-1487-7</doi><tpages>14</tpages></addata></record> |
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| ispartof | Planta, 2005-08, Vol.221 (6), p.776-789 |
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| language | eng |
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| subjects | 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase - metabolism Agronomy. Soil science and plant productions Amino Acid Sequence amino acid sequences Antibodies Antiserum Biological and medical sciences Centrifugation Cytoplasm Economic plant physiology Flowering, floral biology, reproduction patterns Fluorescent antibody techniques Fundamental and applied biological sciences. Psychology Gels Growth and development Isoenzymes methionine methionine synthase methyltransferases microtubules Molecular Sequence Data Nicotiana - enzymology Nicotiana - ultrastructure Nicotiana tabacum Organelles plant biochemistry Plant cells Plant Proteins Pollen Pollen - enzymology Pollen - ultrastructure Pollen tubes polypeptides Protein isoforms Secretory Vesicles - enzymology Secretory Vesicles - ultrastructure sequence alignment Sequence Homology, Amino Acid tobacco Western blotting |
| title | methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes |
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