Oxidized low‐density lipoprotein‐induced expression of ABCA1 in blood monocytes precedes coronary atherosclerosis and is associated with plaque complexity in hypercholesterolemic pigs

Background: Elevated oxidized low‐density lipoprotein (oxLDL) is associated with atherosclerosis and high cardiovascular risk. Previously, we identified 18 genes in coronary plaque macrophages of hypercholesterolemic pigs that correlated with plaque oxLDL. Objective: To determine which of these gene...

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Bibliographic Details
Published in:Journal of thrombosis and haemostasis 2007-12, Vol.5 (12), p.2529-2536
Main Authors: GEERAERT, B., DE KEYZER, D., DAVEY, P. C., CROMBÉ, F., BENHABILÈS, N., HOLVOET, P.
Format: Article
Language:English
Subjects:
Animals
atherosclerosis
ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - metabolism
biomarker
Biomarkers - metabolism
Cells, Cultured
Coronary Artery Disease - blood
Coronary Artery Disease - genetics
Coronary Artery Disease - metabolism
Coronary Artery Disease - pathology
Diet, Atherogenic
Disease Models, Animal
Disease Progression
Gene Expression Regulation
Humans
Hypercholesterolemia - blood
Hypercholesterolemia - complications
Hypercholesterolemia - genetics
Hypercholesterolemia - metabolism
Hypercholesterolemia - pathology
Lipid Metabolism
Lipoproteins, LDL - blood
Macrophages - metabolism
Macrophages - pathology
monocyte
Monocytes - enzymology
Monocytes - metabolism
Monocytes - pathology
oxidized low‐density lipoprotein
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Stearoyl-CoA Desaturase - genetics
Stearoyl-CoA Desaturase - metabolism
Swine
Swine, Miniature
Up-Regulation
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Description
Summary:Background: Elevated oxidized low‐density lipoprotein (oxLDL) is associated with atherosclerosis and high cardiovascular risk. Previously, we identified 18 genes in coronary plaque macrophages of hypercholesterolemic pigs that correlated with plaque oxLDL. Objective: To determine which of these genes were differentially expressed in blood monocytes and correlated with blood and plaque oxLDL and with plaque complexity. Methods: RNA expression in monocytes of 27 hypercholesterolemic and 12 control pigs was analyzed with quantitative real‐time polymerase chain reaction. Results: Five of 12 genes with detectable expression in monocytes were overexpressed (at P 
ISSN:1538-7933
1538-7836
1538-7836
DOI:10.1111/j.1538-7836.2007.02786.x