Acidic Proline-rich Protein Db and Caries in Young Children

Polymorphic, acidic proline-rich proteins (PRPs) in saliva influence the attachment of bacteria associated with caries. Our aims were to detect one of three acidic PRP alleles of the PRH1 locus (Db) using polymerase chain-reaction (PCR) on genomic DNA, and to determine its association with caries. D...

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Bibliographic Details
Published in:Journal of dental research 2007-12, Vol.86 (12), p.1176-1180
Main Authors: Zakhary, G.M., Clark, R.M., Bidichandani, S.I., Owen, W.L., Slayton, R.L., Levine, M.
Format: Article
Language:English
Subjects:
African Americans - genetics
Base Sequence
Case-Control Studies
Chi-Square Distribution
Child
Colony Count, Microbial
Dental Caries - ethnology
Dental Caries - genetics
Dental Caries - microbiology
Dentistry
DMF Index
European Continental Ancestry Group - genetics
Female
Gene Frequency
Humans
Male
Molecular Sequence Data
Peptides - genetics
Peptides - metabolism
Proline-Rich Protein Domains
Saliva - metabolism
Salivary Proline-Rich Proteins
Salivary Proteins and Peptides - genetics
Sequence Analysis, DNA
Social Class
Streptococcus mutans - isolation & purification
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Summary:Polymorphic, acidic proline-rich proteins (PRPs) in saliva influence the attachment of bacteria associated with caries. Our aims were to detect one of three acidic PRP alleles of the PRH1 locus (Db) using polymerase chain-reaction (PCR) on genomic DNA, and to determine its association with caries. DNA was obtained from buccal swabs from Caucasian and African-American children, and their caries experience was recorded. PCR primers designed around exon 3 of the PRH1 locus gave a 416-base product representing Db and a 353-base product representing the other two alleles (Pa or Pif). In Caucasians, Db gene frequency was 14%, similar to Db protein from parotid saliva. In African-Americans, however, it was 37%, 18% lower than Db from parotid saliva (reported previously). Compared with African-Americans, all Caucasians had significantly greater Streptococcus mutans colonization, but only Db-negative Caucasians had significantly more caries. Alleles linked to Db may explain racial differences in caries experience.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910708601207