Inhibition of Hydrogen Peroxide, Nitric Oxide and TNF-α Production in Peritoneal Macrophages by Ethyl Acetate Fraction from Alchornea glandulosa

The effects of Alchornea glandulosa ethyl acetate fraction (AGF) on hydrogen peroxide (H2O2), nitric oxide (NO) and tumor necrosis factor-α (TNF-α) production in peritoneal macrophages activated with lipopolysaccharide (LPS) or phorbol myristate acetate (PMA) were investigated. Analysis by thin laye...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2005, Vol.28(9), pp.1726-1730
Main Authors: Lopes, Flávia Cristine Mascia, Calvo, Tamara Regina, Vilegas, Wagner, Carlos, Iracilda Zeppone
Format: Article
Language:English
Subjects:
Acetates
Alchornea
Alchornea glandulosa
Animals
anti-inflammatory activity
Cell Survival - drug effects
Chromatography, Thin Layer
Euphorbiaceae - chemistry
flavonoid
hydrogen peroxide
Hydrogen Peroxide - antagonists & inhibitors
Hydrogen Peroxide - metabolism
Immunologic Factors - pharmacology
Macrophages, Peritoneal - metabolism
Mice
nitric oxide
Nitric Oxide - biosynthesis
Plant Extracts
Plant Leaves - chemistry
Solvents
TNF-α
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:The effects of Alchornea glandulosa ethyl acetate fraction (AGF) on hydrogen peroxide (H2O2), nitric oxide (NO) and tumor necrosis factor-α (TNF-α) production in peritoneal macrophages activated with lipopolysaccharide (LPS) or phorbol myristate acetate (PMA) were investigated. Analysis by thin layer chromatography (TLC) of AGF showed several constituents, including flavonoids, which may have anti-inflammatory activity. Inhibitory effects of the fraction in H2O2 and NO production ranged from 8.59±7.84% to 70.56±4.16% and from 16.06±3.65% to 38.73±3.90%, respectively. The TNF-α production was only partially inhibited in the tested concentrations (12.21±6.23%—15.16±0.96%). According to these results, it is suggested that AGF has anti-inflammatory activity. This medicinal plant may have therapeutic potential in the control of inflammatory disorders.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.28.1726