Immunolocalization of collagen types in the subsynovial connective tissue within the carpal tunnel in humans

The tenosynovium within the carpal tunnel consists of a single layer of synovial cells, which lines the bursae within the carpal tunnel, and the subsynovial connective tissue (SSCT), which contains the tendon vasculature and other structural elements. In this study, we used immunogold labeling to lo...

Full description

Saved in:
Bibliographic Details
Published in:Journal of orthopaedic research 2005-09, Vol.23 (5), p.1226-1231
Main Authors: Oh, Jinrok, Zhao, Chunfeng, Amadio, Peter C., An, Kai-Nan, Zobitz, Mark E., Wold, Lester E.
Format: Article
Language:English
Subjects:
Aged
Carpal tunnel syndrome
Carpal Tunnel Syndrome - metabolism
Carpal Tunnel Syndrome - pathology
Collagen
Collagen - analysis
Collagen Type I - analysis
Collagen Type III - analysis
Collagen Type VI - analysis
Connective Tissue - chemistry
Connective Tissue - ultrastructure
Humans
Immunogold
Immunohistochemistry
Microscopy, Electron, Transmission
Middle Aged
Tendons - chemistry
Tendons - ultrastructure
Tenosynovium
Transmission electron microscopy (TEM)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The tenosynovium within the carpal tunnel consists of a single layer of synovial cells, which lines the bursae within the carpal tunnel, and the subsynovial connective tissue (SSCT), which contains the tendon vasculature and other structural elements. In this study, we used immunogold labeling to localize collagen types within the SSCT in three cadaver specimens and three patients with carpal tunnel syndrome. Positive labeling for collagen types I, III and VI was found with immunoelectron microscopy. Collagen types I and III were codistributed within the SSCT. Type VI was primarily located in microfibrillar structures between collagen bundles, between elastin and collagen bundles and between collagen bundles and cells. There was no difference in the distribution of collagen types when comparing cadaver specimens and carpal tunnel patients.
ISSN:0736-0266
1554-527X
DOI:10.1016/j.orthres.2005.02.009