Mesenchymal Stem Cells Derived from CD133‐Positive Cells in Mobilized Peripheral Blood and Cord Blood: Proliferation, Oct4 Expression, and Plasticity

In this study, we used a common procedure to assess the potential of mobilized peripheral blood (MPB) and umbilical cord blood (UCB) as sources of mesenchymal stem cells (MSCs) in comparison with bone marrow (BM). We tested three methods: plastic adhesion supplemented with 5% of BM‐MSC conditioned m...

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Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Ohio), 2005-09, Vol.23 (8), p.1105-1112
Main Authors: Tondreau, Tatiana, Meuleman, Nathalie, Delforge, Alain, Dejeneffe, Marielle, Leroy, Rita, Massy, Martine, Mortier, Christine, Bron, Dominique, Lagneaux, Laurence
Format: Article
Language:English
Subjects:
AC133 Antigen
Adipocytes - cytology
Antigens, CD - analysis
Biomarkers - analysis
Blood Cells - cytology
Blood Cells - metabolism
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
Cell Differentiation
Cell Lineage
Cell Proliferation
Cell Separation
Cells, Cultured
Colony-Forming Units Assay
Fetal Blood - cytology
Flow Cytometry
Glycoproteins - analysis
Humans
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Neurons - cytology
Octamer Transcription Factor-3 - metabolism
Osteocytes - cytology
Peptides - analysis
Peripheral blood
RNA, Messenger - metabolism
Umbilical cord blood
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Summary:In this study, we used a common procedure to assess the potential of mobilized peripheral blood (MPB) and umbilical cord blood (UCB) as sources of mesenchymal stem cells (MSCs) in comparison with bone marrow (BM). We tested three methods: plastic adhesion supplemented with 5% of BM‐MSC conditioned medium, unsupplemented plastic adhesion, and selection of CD133‐positive cells. MSCs derived from MPB or UCB are identified by their positive expression of mesenchymal (SH2, SH3) and negative expression of hematopoietic markers (CD14, CD34, CD45, HLA‐DR). We observed that the CD133‐positive cell fraction contains more MSCs with high proliferative potential. Placed in appropriate conditions, these cells proved their capacity to differentiate into adipocytes, osteocytes, chondrocytes, and neuronal/glial cells. MPB‐ and UCB‐MSCs express Oct4, a transcriptional binding factor present in undifferentiated cells with high proliferative capacity. The selection of CD133‐positive cells enabled us to obtain a homogeneous population of MSCs from UCB and MPB. These sources may have a major clinical importance thanks to their easy accessibility.
ISSN:1066-5099
1549-4918
DOI:10.1634/stemcells.2004-0330