Rosmarinic acid content and RAPD analysis of in vitro regenerated basil ( Ocimum americanum) plants

Various in vitro cultures were established from shoot tips of Ocimum americanum seedlings. Rosmarinic acid content of the in vitro produced plants as well as parent plant were determined by HPLC analysis and subjected to RAPD analysis. MS medium with BA at a concentration of 1 mg/l and 0.25 mg/l IAA...

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Bibliographic Details
Published in:Fitoterapia 2005-09, Vol.76 (6), p.525-533
Main Authors: Rady, Mohamed R., Nazif, Naglaa M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Culture Techniques
Cinnamates - metabolism
Culture in vitro
Depsides
DNA Primers
DNA, Plant - analysis
General pharmacology
Humans
Medical sciences
Ocimum - genetics
Ocimum - growth & development
Ocimum - metabolism
Ocimum americanum
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phytotherapy
Plant Shoots - metabolism
Random Amplified Polymorphic DNA Technique
RAPD analysis
Rosmarinic Acid
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Summary:Various in vitro cultures were established from shoot tips of Ocimum americanum seedlings. Rosmarinic acid content of the in vitro produced plants as well as parent plant were determined by HPLC analysis and subjected to RAPD analysis. MS medium with BA at a concentration of 1 mg/l and 0.25 mg/l IAA supports maximum rosmarinic acid production in plants produced from cultures grown on that medium. RAPD analysis revealed 64 scorable bands from four primers, including six polymorphic bands. The band pattern revealed differences between the parent plant and the in vitro regenerated plants. Certain band changes were found in O. americanum plants regenerated in vitro, suggesting the existence of genetic variation that might affect the biochemical synthesis of plants derived from tissue culture.
ISSN:0367-326X
DOI:10.1016/j.fitote.2005.04.001