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Growth and Phenotype of Low-density Nasal Septal Chondrocyte Monolayers

To analyze the growth patterns and differentiation of human septal chondrocyte monolayers of different seeding densities. Chondrocytes from 8 donors were plated at densities ranging from 20,000 cells/cm 2 (high density) to 300 cells/cm 2 (very low density). Confluency, cellularity, and glycosaminogl...

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Bibliographic Details
Published in:Otolaryngology-head and neck surgery 2005-09, Vol.133 (3), p.417-422
Main Authors: Hicks, David L., Sage, August B., Schumacher, Barbara L., Sah, Robert L., Watson, Deborah
Format: Article
Language:English
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Summary:To analyze the growth patterns and differentiation of human septal chondrocyte monolayers of different seeding densities. Chondrocytes from 8 donors were plated at densities ranging from 20,000 cells/cm 2 (high density) to 300 cells/cm 2 (very low density). Confluency, cellularity, and glycosaminoglycan content were determined from days 1 to 15. Confluency was attained at 5.8, 8.3, 11.0, and 14.8 days for high-, intermediate-, low-, and very low–density monolayers, respectively ( P < 0.001). Regression growth curves showed typical lag, logarithmic, and stationary phases. Confluent monolayers attained similar cellularity (power = 0.94) and differentiation (power = 0.88), regardless of initial density. Human septal chondrocyte monolayers reach confluency from very low initial densities. Growth patterns, cellularity, and differentiation are similar to other starting densities. Very low–density monolayers expanded cell number 838-fold in 1 passage and therefore are sufficient for tissue-engineering purposes. This is important because of the requirement of maintaining differentiation and the limitation of small tissue harvest specimens.
ISSN:0194-5998
1097-6817
DOI:10.1016/j.otohns.2005.03.084