A study on the photodynamic properties of chlorophyll derivatives using human hepatocellular carcinoma cells

Photodynamic therapy (PDT) is an alternative anticancer treatment in which direct tumor-cell killing results from selective accumulation of photosensitizers in the tumor sites and phototoxicity occurs when light-activated photosensitizers transfer the energy to oxygen nearby to produce singlet oxyge...

Full description

Saved in:
Bibliographic Details
Published in:Photochemical & photobiological sciences 2007-12, Vol.6 (12), p.1341-1348
Main Authors: Li, Wen-Tyng, Tsao, Hsin-Wei, Chen, Ying-Ying, Cheng, Shih-Wei, Hsu, Yih-Chih
Format: Article
Language:English
Subjects:
Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - radiotherapy
Caspase 3 - metabolism
Caspase 9 - metabolism
Cell Line, Tumor
Chlorophyll - analogs & derivatives
Chlorophyll - pharmacology
Collagen Type XI - metabolism
Cytochromes c - metabolism
Humans
Photochemotherapy
Photosensitizing Agents - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c381t-b0d82429cc4aacabab3901f559aa2c6385a8462c9a6a419db79c6ae811f61413
cites cdi_FETCH-LOGICAL-c381t-b0d82429cc4aacabab3901f559aa2c6385a8462c9a6a419db79c6ae811f61413
container_end_page 1348
container_issue 12
container_start_page 1341
container_title Photochemical & photobiological sciences
container_volume 6
creator Li, Wen-Tyng
Tsao, Hsin-Wei
Chen, Ying-Ying
Cheng, Shih-Wei
Hsu, Yih-Chih
description Photodynamic therapy (PDT) is an alternative anticancer treatment in which direct tumor-cell killing results from selective accumulation of photosensitizers in the tumor sites and phototoxicity occurs when light-activated photosensitizers transfer the energy to oxygen nearby to produce singlet oxygen. The objective of this study was to investigate the effects of PDT using chlorophyll derivatives such as pheophytin a (phe a), pheophytin b (phe b), pheophorbide a (pho a) and pheophorbide b (pho b) as the photosensitizers, and the 660 nm light-emitting diodes (LEDs) irradiation on human hepatocellular carcinoma cells (HuH-7). The drug concentration-dependent inhibition of HuH-7 cell viability was studied under LEDs irradiation (10 mW cm(-2)) at radiant exposure of 5.1 and 10.2 J cm(-2) by MTT assay. Significant inhibition of the survival of HuH-7 cells (
doi_str_mv 10.1039/b704539e
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68559145</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68559145</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-b0d82429cc4aacabab3901f559aa2c6385a8462c9a6a419db79c6ae811f61413</originalsourceid><addsrcrecordid>eNpFkEtLxDAUhYMojo6Cv0CyEjfVpE3TZjkMvmDAzSzclds0tZG0qXkM9N_bYUZd3cs5h8PhQ-iGkgdKMvFYF4TlmVAn6IKygiWCiPT0788_FujS-y9CaM54cY4WtCSMM0EvkFlhH2IzYTvg0Ck8djbYZhqg1xKPzo7KBa08ti2WnbGz0E3G4EY5vYOgd7MVvR4-cRd7GHCnRghWKmOiAYclOKkH2wPeS_4KnbVgvLo-3iXaPj9t16_J5v3lbb3aJDIraUhq0pQpS4WUDEBCDXUmCG3zXACkkmdlDiXjqRTAgVHR1IWQHFRJacspo9kS3R1q5_3fUflQ9drvB8CgbPQVL-cqOgNbovtDUDrrvVNtNTrdg5sqSqo92OoX7By9PXbGulfNf_BIMvsBxnZ12A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68559145</pqid></control><display><type>article</type><title>A study on the photodynamic properties of chlorophyll derivatives using human hepatocellular carcinoma cells</title><source>Springer Link</source><source>Royal Society of Chemistry: Jisc Collections: Journals Archive 1841-2007 (2019-2023)</source><creator>Li, Wen-Tyng ; Tsao, Hsin-Wei ; Chen, Ying-Ying ; Cheng, Shih-Wei ; Hsu, Yih-Chih</creator><creatorcontrib>Li, Wen-Tyng ; Tsao, Hsin-Wei ; Chen, Ying-Ying ; Cheng, Shih-Wei ; Hsu, Yih-Chih</creatorcontrib><description>Photodynamic therapy (PDT) is an alternative anticancer treatment in which direct tumor-cell killing results from selective accumulation of photosensitizers in the tumor sites and phototoxicity occurs when light-activated photosensitizers transfer the energy to oxygen nearby to produce singlet oxygen. The objective of this study was to investigate the effects of PDT using chlorophyll derivatives such as pheophytin a (phe a), pheophytin b (phe b), pheophorbide a (pho a) and pheophorbide b (pho b) as the photosensitizers, and the 660 nm light-emitting diodes (LEDs) irradiation on human hepatocellular carcinoma cells (HuH-7). The drug concentration-dependent inhibition of HuH-7 cell viability was studied under LEDs irradiation (10 mW cm(-2)) at radiant exposure of 5.1 and 10.2 J cm(-2) by MTT assay. Significant inhibition of the survival of HuH-7 cells (&lt;10%) was observed when an irradiation dose of 10.2 J cm(-2) combined with the concentration of 0.5 microg ml(-1) of phe a, 0.125 microg ml(-1) of pho a, 0.25 microg ml(-1) of phe b, and 0.125 microg ml(-1) of pho b were applied. The results from Annexin V-propidium iodide staining revealed that phe a, phe b, pho a and pho b could induce cell death in HuH-7 cells predominantly via a necrotic process. The results from immunoblot analyses exhibited that chlorophyll derivative-mediated PDT initiated cytochrome c release, caspase-9 and caspase-3 activation, followed by poly ADP-ribose polymerase (PARP) cleavage. Thus, apoptosis also occurred in HuH-7 cells after PDT treatment, and the execution of the apoptotic process may be initiated from the loss of mitochondrial function. Our findings demonstrate that both apoptosis and necrosis can be induced in HuH-7 cells after PDT using phe a, phe b, pho a and pho b and LEDs.</description><identifier>ISSN: 1474-905X</identifier><identifier>EISSN: 1474-9092</identifier><identifier>DOI: 10.1039/b704539e</identifier><identifier>PMID: 18046491</identifier><language>eng</language><publisher>England</publisher><subject>Carcinoma, Hepatocellular - drug therapy ; Carcinoma, Hepatocellular - radiotherapy ; Caspase 3 - metabolism ; Caspase 9 - metabolism ; Cell Line, Tumor ; Chlorophyll - analogs &amp; derivatives ; Chlorophyll - pharmacology ; Collagen Type XI - metabolism ; Cytochromes c - metabolism ; Humans ; Photochemotherapy ; Photosensitizing Agents - pharmacology</subject><ispartof>Photochemical &amp; photobiological sciences, 2007-12, Vol.6 (12), p.1341-1348</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-b0d82429cc4aacabab3901f559aa2c6385a8462c9a6a419db79c6ae811f61413</citedby><cites>FETCH-LOGICAL-c381t-b0d82429cc4aacabab3901f559aa2c6385a8462c9a6a419db79c6ae811f61413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18046491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Wen-Tyng</creatorcontrib><creatorcontrib>Tsao, Hsin-Wei</creatorcontrib><creatorcontrib>Chen, Ying-Ying</creatorcontrib><creatorcontrib>Cheng, Shih-Wei</creatorcontrib><creatorcontrib>Hsu, Yih-Chih</creatorcontrib><title>A study on the photodynamic properties of chlorophyll derivatives using human hepatocellular carcinoma cells</title><title>Photochemical &amp; photobiological sciences</title><addtitle>Photochem Photobiol Sci</addtitle><description>Photodynamic therapy (PDT) is an alternative anticancer treatment in which direct tumor-cell killing results from selective accumulation of photosensitizers in the tumor sites and phototoxicity occurs when light-activated photosensitizers transfer the energy to oxygen nearby to produce singlet oxygen. The objective of this study was to investigate the effects of PDT using chlorophyll derivatives such as pheophytin a (phe a), pheophytin b (phe b), pheophorbide a (pho a) and pheophorbide b (pho b) as the photosensitizers, and the 660 nm light-emitting diodes (LEDs) irradiation on human hepatocellular carcinoma cells (HuH-7). The drug concentration-dependent inhibition of HuH-7 cell viability was studied under LEDs irradiation (10 mW cm(-2)) at radiant exposure of 5.1 and 10.2 J cm(-2) by MTT assay. Significant inhibition of the survival of HuH-7 cells (&lt;10%) was observed when an irradiation dose of 10.2 J cm(-2) combined with the concentration of 0.5 microg ml(-1) of phe a, 0.125 microg ml(-1) of pho a, 0.25 microg ml(-1) of phe b, and 0.125 microg ml(-1) of pho b were applied. The results from Annexin V-propidium iodide staining revealed that phe a, phe b, pho a and pho b could induce cell death in HuH-7 cells predominantly via a necrotic process. The results from immunoblot analyses exhibited that chlorophyll derivative-mediated PDT initiated cytochrome c release, caspase-9 and caspase-3 activation, followed by poly ADP-ribose polymerase (PARP) cleavage. Thus, apoptosis also occurred in HuH-7 cells after PDT treatment, and the execution of the apoptotic process may be initiated from the loss of mitochondrial function. Our findings demonstrate that both apoptosis and necrosis can be induced in HuH-7 cells after PDT using phe a, phe b, pho a and pho b and LEDs.</description><subject>Carcinoma, Hepatocellular - drug therapy</subject><subject>Carcinoma, Hepatocellular - radiotherapy</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase 9 - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Chlorophyll - analogs &amp; derivatives</subject><subject>Chlorophyll - pharmacology</subject><subject>Collagen Type XI - metabolism</subject><subject>Cytochromes c - metabolism</subject><subject>Humans</subject><subject>Photochemotherapy</subject><subject>Photosensitizing Agents - pharmacology</subject><issn>1474-905X</issn><issn>1474-9092</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNpFkEtLxDAUhYMojo6Cv0CyEjfVpE3TZjkMvmDAzSzclds0tZG0qXkM9N_bYUZd3cs5h8PhQ-iGkgdKMvFYF4TlmVAn6IKygiWCiPT0788_FujS-y9CaM54cY4WtCSMM0EvkFlhH2IzYTvg0Ck8djbYZhqg1xKPzo7KBa08ti2WnbGz0E3G4EY5vYOgd7MVvR4-cRd7GHCnRghWKmOiAYclOKkH2wPeS_4KnbVgvLo-3iXaPj9t16_J5v3lbb3aJDIraUhq0pQpS4WUDEBCDXUmCG3zXACkkmdlDiXjqRTAgVHR1IWQHFRJacspo9kS3R1q5_3fUflQ9drvB8CgbPQVL-cqOgNbovtDUDrrvVNtNTrdg5sqSqo92OoX7By9PXbGulfNf_BIMvsBxnZ12A</recordid><startdate>200712</startdate><enddate>200712</enddate><creator>Li, Wen-Tyng</creator><creator>Tsao, Hsin-Wei</creator><creator>Chen, Ying-Ying</creator><creator>Cheng, Shih-Wei</creator><creator>Hsu, Yih-Chih</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200712</creationdate><title>A study on the photodynamic properties of chlorophyll derivatives using human hepatocellular carcinoma cells</title><author>Li, Wen-Tyng ; Tsao, Hsin-Wei ; Chen, Ying-Ying ; Cheng, Shih-Wei ; Hsu, Yih-Chih</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-b0d82429cc4aacabab3901f559aa2c6385a8462c9a6a419db79c6ae811f61413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Carcinoma, Hepatocellular - drug therapy</topic><topic>Carcinoma, Hepatocellular - radiotherapy</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase 9 - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Chlorophyll - analogs &amp; derivatives</topic><topic>Chlorophyll - pharmacology</topic><topic>Collagen Type XI - metabolism</topic><topic>Cytochromes c - metabolism</topic><topic>Humans</topic><topic>Photochemotherapy</topic><topic>Photosensitizing Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Wen-Tyng</creatorcontrib><creatorcontrib>Tsao, Hsin-Wei</creatorcontrib><creatorcontrib>Chen, Ying-Ying</creatorcontrib><creatorcontrib>Cheng, Shih-Wei</creatorcontrib><creatorcontrib>Hsu, Yih-Chih</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photochemical &amp; photobiological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Wen-Tyng</au><au>Tsao, Hsin-Wei</au><au>Chen, Ying-Ying</au><au>Cheng, Shih-Wei</au><au>Hsu, Yih-Chih</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A study on the photodynamic properties of chlorophyll derivatives using human hepatocellular carcinoma cells</atitle><jtitle>Photochemical &amp; photobiological sciences</jtitle><addtitle>Photochem Photobiol Sci</addtitle><date>2007-12</date><risdate>2007</risdate><volume>6</volume><issue>12</issue><spage>1341</spage><epage>1348</epage><pages>1341-1348</pages><issn>1474-905X</issn><eissn>1474-9092</eissn><abstract>Photodynamic therapy (PDT) is an alternative anticancer treatment in which direct tumor-cell killing results from selective accumulation of photosensitizers in the tumor sites and phototoxicity occurs when light-activated photosensitizers transfer the energy to oxygen nearby to produce singlet oxygen. The objective of this study was to investigate the effects of PDT using chlorophyll derivatives such as pheophytin a (phe a), pheophytin b (phe b), pheophorbide a (pho a) and pheophorbide b (pho b) as the photosensitizers, and the 660 nm light-emitting diodes (LEDs) irradiation on human hepatocellular carcinoma cells (HuH-7). The drug concentration-dependent inhibition of HuH-7 cell viability was studied under LEDs irradiation (10 mW cm(-2)) at radiant exposure of 5.1 and 10.2 J cm(-2) by MTT assay. Significant inhibition of the survival of HuH-7 cells (&lt;10%) was observed when an irradiation dose of 10.2 J cm(-2) combined with the concentration of 0.5 microg ml(-1) of phe a, 0.125 microg ml(-1) of pho a, 0.25 microg ml(-1) of phe b, and 0.125 microg ml(-1) of pho b were applied. The results from Annexin V-propidium iodide staining revealed that phe a, phe b, pho a and pho b could induce cell death in HuH-7 cells predominantly via a necrotic process. The results from immunoblot analyses exhibited that chlorophyll derivative-mediated PDT initiated cytochrome c release, caspase-9 and caspase-3 activation, followed by poly ADP-ribose polymerase (PARP) cleavage. Thus, apoptosis also occurred in HuH-7 cells after PDT treatment, and the execution of the apoptotic process may be initiated from the loss of mitochondrial function. Our findings demonstrate that both apoptosis and necrosis can be induced in HuH-7 cells after PDT using phe a, phe b, pho a and pho b and LEDs.</abstract><cop>England</cop><pmid>18046491</pmid><doi>10.1039/b704539e</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1474-905X
ispartof Photochemical & photobiological sciences, 2007-12, Vol.6 (12), p.1341-1348
issn 1474-905X
1474-9092
language eng
recordid cdi_proquest_miscellaneous_68559145
source Springer Link; Royal Society of Chemistry: Jisc Collections: Journals Archive 1841-2007 (2019-2023)
subjects Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - radiotherapy
Caspase 3 - metabolism
Caspase 9 - metabolism
Cell Line, Tumor
Chlorophyll - analogs & derivatives
Chlorophyll - pharmacology
Collagen Type XI - metabolism
Cytochromes c - metabolism
Humans
Photochemotherapy
Photosensitizing Agents - pharmacology
title A study on the photodynamic properties of chlorophyll derivatives using human hepatocellular carcinoma cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T12%3A40%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20study%20on%20the%20photodynamic%20properties%20of%20chlorophyll%20derivatives%20using%20human%20hepatocellular%20carcinoma%20cells&rft.jtitle=Photochemical%20&%20photobiological%20sciences&rft.au=Li,%20Wen-Tyng&rft.date=2007-12&rft.volume=6&rft.issue=12&rft.spage=1341&rft.epage=1348&rft.pages=1341-1348&rft.issn=1474-905X&rft.eissn=1474-9092&rft_id=info:doi/10.1039/b704539e&rft_dat=%3Cproquest_cross%3E68559145%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c381t-b0d82429cc4aacabab3901f559aa2c6385a8462c9a6a419db79c6ae811f61413%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=68559145&rft_id=info:pmid/18046491&rfr_iscdi=true