Histologic findings after gastrocystoplasty in rabbits

The aim of this study was to investigate the long-term histologic changes after bladder augmentation with gastric segment in an animal subject. Gastrocystoplasty was performed in 13 young, 3-month-old male rabbits. Open biopsies were taken from the native bladder and the gastric segment preoperative...

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Bibliographic Details
Published in:Journal of pediatric surgery 2005-09, Vol.40 (9), p.1470-1474
Main Authors: Vajda, Peter, Pinter, Andrew B., Magyarlaki, Tamas, Vastyan, Attila M., Juhasz, Zsolt, Oberritter, Zsolt, Fathi, Khaled
Format: Article
Language:English
Subjects:
Animals
Atrophy
Cell Transformation, Neoplastic
Gastric Mucosa - growth & development
Gastric Mucosa - pathology
Hyperplasia
Inflammation
Male
Postoperative Complications
Rabbits
Risk Factors
Stomach - pathology
Stomach - transplantation
Treatment Outcome
Urinary Bladder - pathology
Urinary Bladder - surgery
Urinary Bladder Neoplasms - etiology
Urinary Incontinence - surgery
Urologic Surgical Procedures - methods
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Summary:The aim of this study was to investigate the long-term histologic changes after bladder augmentation with gastric segment in an animal subject. Gastrocystoplasty was performed in 13 young, 3-month-old male rabbits. Open biopsies were taken from the native bladder and the gastric segment preoperatively and at 3, 6, and 12 months postoperatively. Sections were examined with H&E and periodic acid–Schiff (PAS) staining. Indirect immune peroxidase method was additionally applied to detect the carcinoembrionic antigen, the proliferative activity, and the gene for the tumor protein p53 in the epithelium. On the native bladder, at the 3-month follow-up, polyps, mucosal edema, submucosal fibrosis, and squamous cell metaplasia were detected, which did not change during the follow-up. On the gastric segment, at the 3-month follow-up, parietal cell hyperplasia and inflammatory mucosal overgrowth were detected; at the 6-month follow-up, inflammation or atrophy of the gastric mucosa and colonic-type metaplasia was found. These alterations remained unchanged during later course of follow-up. Neither dysplasia nor malignancy was observed during the 12-month follow-up. The present study supports the clinical observations of low cancer risk after gastrocystoplasty and may indicate different effect of gastric secretion on uroepithelium and that of urine on gastric mucosa.
ISSN:0022-3468
1531-5037
DOI:10.1016/j.jpedsurg.2005.05.047