Characterization of matrix metalloproteinases in denervated muscle

In a nerve crush model of denervation, we examined muscle matrix metalloproteinase (MMP) expression, localization and activity. In normal muscle, MMP mRNA levels were low, and immunohistochemically MMPs were distributed around the muscle fibre with MMPs‐3, ‐7 and ‐9 also staining at the neuromuscula...

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Bibliographic Details
Published in:Neuropathology and applied neurobiology 2005-10, Vol.31 (5), p.545-555
Main Authors: Demestre, M., Orth, M., Wells, G. M., Gearing, A. J., Hughes, R. A. C., Gregson, N. A.
Format: Article
Language:English
Subjects:
72 kDa gelatinase
Animals
Biological and medical sciences
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Immunohistochemistry
macrophage metalloelastase
Male
matrix metalloproteinases
Matrix Metalloproteinases - biosynthesis
Medical sciences
MT1-MMP
Muscle Denervation
Muscle, Skeletal - enzymology
Muscle, Skeletal - innervation
Muscle, Skeletal - pathology
Nerve Crush
nerve regeneration
Nerve Regeneration - physiology
Neurology
neuropathy
Rats
Rats, Inbred Lew
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Sciatic Nerve - injuries
Tumors of the nervous system. Phacomatoses
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Summary:In a nerve crush model of denervation, we examined muscle matrix metalloproteinase (MMP) expression, localization and activity. In normal muscle, MMP mRNA levels were low, and immunohistochemically MMPs were distributed around the muscle fibre with MMPs‐3, ‐7 and ‐9 also staining at the neuromuscular junction. Seven days after nerve crush, muscle MMP immunoreactivity, especially MMP‐12 and MMP‐14, became irregularly distributed. At 20 days reinnervation of the muscle was observed, and some restitution of the normal pattern of immunoreactivity was noted concomitant with a higher level of MMP mRNA expression. In situ zymography showed that MMP activity was very weak in normal muscle whereas it was increased up to 40 days following denervation. Our results suggest that MMPs in muscle are involved in the tissue changes following denervation. Further experiments are required to test the hypothesis that MMP inhibition may be beneficial in protecting muscle from excessive remodelling following denervation and therefore improve reinnervation.
ISSN:0305-1846
1365-2990
DOI:10.1111/j.1365-2990.2005.00676.x