Antigen microarray profiling of autoantibodies in rheumatoid arthritis

Objective Because rheumatoid arthritis (RA) is a heterogeneous autoimmune disease in terms of disease manifestations, clinical outcomes, and therapeutic responses, we developed and applied a novel antigen microarray technology to identify distinct serum antibody profiles in patients with RA. Methods...

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Bibliographic Details
Published in:Arthritis and rheumatism 2005-09, Vol.52 (9), p.2645-2655
Main Authors: Hueber, Wolfgang, Kidd, Brian A., Tomooka, Beren H., Lee, Byung J., Bruce, Bonnie, Fries, James F., Sønderstrup, Grete, Monach, Paul, Drijfhout, Jan W., van Venrooij, Walther J., Utz, Paul J., Genovese, Mark C., Robinson, William H.
Format: Article
Language:English
Subjects:
Algorithms
Antigens - immunology
Antigens - metabolism
Arthritis, Rheumatoid - diagnosis
Arthritis, Rheumatoid - immunology
Arthritis, Rheumatoid - metabolism
Autoantibodies - analysis
Biological and medical sciences
Cluster Analysis
Diseases of the osteoarticular system
Early Diagnosis
Humans
Inflammatory joint diseases
Medical sciences
Protein Array Analysis - methods
Proteome - immunology
Proteome - metabolism
Proteomics
Synovial Membrane - immunology
Synovial Membrane - metabolism
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Summary:Objective Because rheumatoid arthritis (RA) is a heterogeneous autoimmune disease in terms of disease manifestations, clinical outcomes, and therapeutic responses, we developed and applied a novel antigen microarray technology to identify distinct serum antibody profiles in patients with RA. Methods Synovial proteome microarrays, containing 225 peptides and proteins that represent candidate and control antigens, were developed. These arrays were used to profile autoantibodies in randomly selected sera from 2 different cohorts of patients: the Stanford Arthritis Center inception cohort, comprising 18 patients with established RA and 38 controls, and the Arthritis, Rheumatism, and Aging Medical Information System cohort, comprising 58 patients with a clinical diagnosis of RA of
ISSN:0004-3591
1529-0131
DOI:10.1002/art.21269