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An acid dissociation bridging ELISA for detection of antibodies directed against therapeutic proteins in the presence of antigen

Bridging Enzyme-Linked Immunosorbent Assay (ELISA) is commonly used in detection of antibodies directed against therapeutic proteins. Advantages of the bridging ELISA include the capability to detect antibodies regardless of their isotype or the species of origin. However, detection of antibodies ca...

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Published in:	Journal of immunological methods 2005-09, Vol.304 (1), p.189-195
Main Authors:	Patton, Aaron, Mullenix, Michael C., Swanson, Steven J., Koren, Eugen
Format:	Article
Language:	English
Subjects:	Acid dissociation Antibodies - analysis Antibody assay Antigen interference Antigens - immunology Biological and medical sciences Calibration ELISA Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Molecular immunology Proteins - immunology Proteins - therapeutic use Techniques Therapeutic proteins
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container_end_page	195
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container_title	Journal of immunological methods
container_volume	304
creator	Patton, Aaron Mullenix, Michael C. Swanson, Steven J. Koren, Eugen
description	Bridging Enzyme-Linked Immunosorbent Assay (ELISA) is commonly used in detection of antibodies directed against therapeutic proteins. Advantages of the bridging ELISA include the capability to detect antibodies regardless of their isotype or the species of origin. However, detection of antibodies can be difficult, if not impossible, in the presence of high levels of the antigen in the sample matrix. This protocol describes a bridging ELISA that uses a covalently coupled high density antigen surface combined with an acid dissociation step to allow for antibody detection in the presence of antigen in human serum.
doi_str_mv	10.1016/j.jim.2005.06.014
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Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Molecular immunology</subject><subject>Proteins - immunology</subject><subject>Proteins - therapeutic use</subject><subject>Techniques</subject><subject>Therapeutic proteins</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkUFvEzEQhS0EomnhB3BBvtDbLjO7a3tXnKKqlEqRONCeLa89Gxwl3mA7SNz46XVIUG9wsvzme0-jeYy9Q6gRUH7c1Bu_qxsAUYOsAbsXbIG9aio1gHjJFgBNU6ESwwW7TGkDAAgSXrMLlAiqEf2C_V4Gbqx33PmUZutN9nPgY_Ru7cOa367uvy35NEfuKJP9M5wnbkL24-w8peKLRSfHzdr4kDLP3ymaPR2yt3wf50xF5T4c9fKnRMHS34w1hTfs1WS2id6e3yv2-Pn24eZLtfp6d3-zXFW2U32uhtapqQOnDBrXKgFKCCUd9UqKrmutGa1CAts7QCGEGccBW8BB2rG3Dqm9Yten3LLTjwOlrHc-WdpuTaD5kLTshRwUNP8FUbVDN8i-gHgCbZxTijTpffQ7E39pBH3sR2906Ucf-9EgdemneN6fww_jjtyz41xIAT6cAZOs2U7RBOvTM6cQGxhE4T6dOCo3--kp6mT98bSnPrSb_T_WeAIRi66K</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>Patton, Aaron</creator><creator>Mullenix, Michael C.</creator><creator>Swanson, Steven J.</creator><creator>Koren, Eugen</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050901</creationdate><title>An acid dissociation bridging ELISA for detection of antibodies directed against therapeutic proteins in the presence of antigen</title><author>Patton, Aaron ; Mullenix, Michael C. ; Swanson, Steven J. ; Koren, Eugen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-93d7f40d7a1ad375075576de8765443cabc71e0c8d01555abb9130196cb8cd1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acid dissociation</topic><topic>Antibodies - analysis</topic><topic>Antibody assay</topic><topic>Antigen interference</topic><topic>Antigens - immunology</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Fundamental and applied biological sciences. 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subjects	Acid dissociation Antibodies - analysis Antibody assay Antigen interference Antigens - immunology Biological and medical sciences Calibration ELISA Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Molecular immunology Proteins - immunology Proteins - therapeutic use Techniques Therapeutic proteins
title	An acid dissociation bridging ELISA for detection of antibodies directed against therapeutic proteins in the presence of antigen
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