Xylitol production from corn fiber and sugarcane bagasse hydrolysates by Candida tropicalis

• Published in: Bioresource technology 2006-10, Vol.97 (15), p.1974-1978
• Main Authors: Rao, R. Sreenivas, Jyothi, Ch. Pavana, Prakasham, R.S., Sarma, P.N., Rao, L. Venkateswar
• Format: Article
• Language: English
• Subjects: Adaptation; Biological and medical sciences; Candida tropicalis; Candida tropicalis - metabolism; Cellulose - metabolism; Corn fiber; Food industries; Fundamental and applied biological sciences. Psychology; Hemicellulose; Hydrolysis; Industrial Microbiology - economics; Industrial Microbiology - methods; Saccharum; Sugarcane bagasse; Use and upgrading of agricultural and food by-products. Biotechnology; Xylitol; Xylitol - biosynthesis; Zea mays - metabolism
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2005.08.015

A natural isolate, Candida tropicalis was tested for xylitol production from corn fiber and sugarcane bagasse hydrolysates. Fermentation of corn fiber and sugarcane bagasse hydrolysate showed xylose uptake and xylitol production, though these were very low, even after hydrolysate neutralization and treatments with activated charcoal and ion exchange resins. Initial xylitol production was found to be 0.43 g/g and 0.45 g/g of xylose utilised with corn fiber and sugarcane bagasse hydrolysate respectively. One of the critical factors for low xylitol production was the presence of inhibitors in these hydrolysates. To simulate influence of hemicellulosic sugar composition on xylitol yield, three different combinations of mixed sugar control experiments, without the presence of any inhibitors, have been performed and the strain produced 0.63 g/g, 0.68 g/g and 0.72 g/g of xylose respectively. To improve yeast growth and xylitol production with these hydrolysates, which contain inhibitors, the cells were adapted by sub culturing in the hydrolysate containing medium for 25 cycles. After adaptation the organism produced more xylitol 0.58 g/g and 0.65 g/g of xylose with corn fiber hydrolysate and sugarcane bagasse hydrolysate respectively.