Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from a...

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Bibliographic Details
Published in:Journal of neurovirology 2006-04, Vol.12 (2), p.146-152
Main Authors: Churchill, Melissa J, Gorry, Paul R, Cowley, Daniel, Lal, Luxshimi, Sonza, Secondo, Purcell, Damian FJ, Thompson, Katherine A, Gabuzda, Dana, McArthur, Justin C, Pardo, Carlos A, Wesselingh, Steven L
Format: Article
Language:English
Subjects:
AIDS Dementia Complex - virology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiviral agents
Astrocytes - virology
Biological and medical sciences
Brain - virology
Cell Nucleus - chemistry
DNA, Viral - analysis
HIV-1 - isolation & purification
HIV-1 - physiology
Human immunodeficiency virus 1
Human viral diseases
Humans
Infectious diseases
Lasers
Macrophages - virology
Medical sciences
Microdissection
Pharmacology. Drug treatments
Polymerase Chain Reaction
Proviruses - physiology
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral diseases of the nervous system
Virus Integration
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Summary:The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.
ISSN:1355-0284
1538-2443
DOI:10.1080/13550280600748946