Landscape phage probes for Salmonella typhimurium

We selected from landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for S. typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA), and the precipit...

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Bibliographic Details
Published in:Journal of microbiological methods 2005-10, Vol.63 (1), p.55-72
Main Authors: Sorokulova, Iryna B., Olsen, Eric V., Chen, I-Hsuan, Fiebor, Ben, Barbaree, James M., Vodyanoy, Vitaly J., Chin, Bryan A., Petrenko, Valery A.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Biosorbent
Detection
Enterobacteriaceae
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Landscape phage
Microbiological Techniques - methods
Microbiology
Monitoring
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Peptide Library
Phage display
Probe
Salmonella typhimurium
Salmonella typhimurium - chemistry
Salmonella typhimurium - isolation & purification
Sensitivity and Specificity
Techniques used in virology
Virology
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Summary:We selected from landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for S. typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA), and the precipitation test. Interaction of representative probes with S. typhimurium was characterized by fluorescence-activated cell sorting (FACS), and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of specific and robust probes for S. typhimurium suitable for long-term use in continuous monitoring devices and biosorbents.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2005.02.019