Iloprost for the attenuation of ischaemia/reperfusion injury in a distant organ

• Published in: Cell biochemistry and function 2006-07, Vol.24 (4), p.341-346
• Main Authors: Aytacoglu, Barlas N., Sucu, Nehir, Tamer, Lulufer, Polat, Ayse, Gul, Ali, Degirmenci, Ulas, Mavioglu, Ilhan, Dikmengil, Murat
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphatases - blood; Adenosine Triphosphatases - metabolism; Animals; Hindlimb - blood supply; Hindlimb - physiopathology; iloprost; Iloprost - pharmacology; ischaemia reperfusion injury; kidney; Kidney Diseases - etiology; Kidney Diseases - metabolism; Kidney Diseases - prevention & control; Male; Malondialdehyde - blood; Malondialdehyde - metabolism; Peroxidase - blood; Peroxidase - metabolism; Protective Agents - pharmacology; Rats; Rats, Wistar; Reperfusion Injury - complications; Reperfusion Injury - drug therapy; Reperfusion Injury - physiopathology
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1223

The objective of this study was to investigate antioxidant and cytoprotective properties of iloprost in a distant organ after ischaemia reperfusion injury. Male Wistar rats were divided into two groups. After application of anesthaesia both hindlimbs were occluded. A 2‐h reperfusion procedure was carried out after 60 min of ischemia. Study group (STU) rats (n=10) received 10 μg kg−1 iloprost in 1 ml of saline from the tail vein 10 min before reperfusion. Control (CON) group rats (n=10) received an equal amount of saline. The rats were sacrificed by injection of a high dose of thiopentone sodium. Blood and tissue samples (right kidneys) were taken for analysis. Differences in malondialdehyde (MDA), myeloperoxidase (MPO), Na+‐K+ ATPase and total antioxidant capacity (TAC) between the groups were analysed. MPO, MDA and TAC levels in the sera of CON and STU groups were 1.60±0.26 U l−1, 11.42±5.23 nmol ml−1, 8.30×10−2± 3.93×10−2 nmol ml−1 h−1 and 1.07±0.11 U l−1, 7.60±1.81 nmol ml−1 and 0.15±3.23×10−2 nmol ml−1 h−1 (p=0.0001, p=0.043 and p=0.0001 respectively). MPO, ATPase and MDA levels in kidneys for CON and STU groups were 1.24±0.58 U g−1, 85.70±52.05 nmol mg−1, 17.90±7.40 nmol ml−1 and 0.78±0.31 U g−1, 195.90±56.13 nmol mg−1 and 10.10±0.99 nmol ml−1 (p=0.046, p=0.0001 and p=0.009 respectively). When given prior to reperfusion, the positive effect of iloprost in the attenuation of distant organ reperfusion injury has been demonstrated. Copyright © 2005 John Wiley & Sons, Ltd.