The combined effects of zafirlukast, prednisone, and inhaled budesonide on IL-13 and IFN-gamma secretion

Therapy for asthma often includes the combined use of glucocorticoids and leukotriene receptor antagonists. The short-term, combined effects of these drugs on cytokine secretion and lymphocyte proliferation are ill-defined. The aim of this study was to analyze allergen and mitogen-induced cytokine s...

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Bibliographic Details
Published in:Journal of clinical immunology 2005-09, Vol.25 (5), p.437-444
Main Authors: Gibbons, Fiona K, Israel, Elliot, Deykin, Aaron, Schaub, Bianca, He, Hong Z, Perkins, David L, Finn, Patricia W
Format: Article
Language:English
Subjects:
Administration, Inhalation
Adolescent
Adult
Aged
Asthma - blood
Asthma - drug therapy
Asthma - immunology
Budesonide - administration & dosage
Budesonide - therapeutic use
Drug Therapy, Combination
Female
Glucocorticoids - administration & dosage
Glucocorticoids - therapeutic use
Glycoproteins
Humans
Interferon-gamma - blood
Interleukin-13 - blood
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - secretion
Leukotriene Antagonists - administration & dosage
Leukotriene Antagonists - therapeutic use
Lymphocyte Activation
Male
Middle Aged
Phytohemagglutinins
Prednisone - administration & dosage
Prednisone - therapeutic use
Tosyl Compounds - administration & dosage
Tosyl Compounds - therapeutic use
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Summary:Therapy for asthma often includes the combined use of glucocorticoids and leukotriene receptor antagonists. The short-term, combined effects of these drugs on cytokine secretion and lymphocyte proliferation are ill-defined. The aim of this study was to analyze allergen and mitogen-induced cytokine secretion and lymphocyte proliferation in asthmatics and to determine the effect of combined therapy on these immune responses. Peripheral blood mononuclear cells were isolated from mild, persistent adult asthmatics (n = 28) and analyzed for cat allergen (Fel d 1) and mitogen (phytohemagglutinin) induced IL-13 and IFN-gamma secretion and lymphocyte proliferation. Samples were analyzed before and after 10 days of therapy with oral zafirlukast, prednisone (0.5 mg/kg/day), and inhaled budesonide (1600 mcg/day). Both Fel d 1 and mitogen stimulation resulted in IL-13 and IFN-gamma secretion. Combination drug therapy resulted in a significant decrease in allergen-induced IFN-gamma secretion (p = 0.018) and allergen-specific lymphocyte proliferation (p = 0.02), while IL-13 secretion was unchanged (p = 0.109). This study indicates a role for Th1 cytokines as well as Th2 cytokines in the allergic response.
ISSN:0271-9142
1573-2592
DOI:10.1007/s10875-005-5625-6