A combination of diagnostic tools for rapid screening of ovine listeriosis

A combined serological and PCR method for the detection of Listeria monocytogenes infection in symptomatic and asymptomatic ovine flocks was evaluated. Seventy-eight milk samples and 157 serum samples were analysed using a L. monocytogenes PCR detection kit and an anti-listeriolysin O IgG immunoassa...

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Bibliographic Details
Published in:Research in veterinary science 2006-10, Vol.81 (2), p.185-189
Main Authors: Amagliani, G., Giammarini, C., Omiccioli, E., Merati, E.G., Pezzotti, G., Filippini, G., Brandi, G., Magnani, M.
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Antibodies, Bacterial - blood
bacterial antigens
Bacterial Toxins - chemistry
blood serum
disease detection
disease diagnosis
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
drug therapy
ELISA
Enzyme-Linked Immunosorbent Assay - veterinary
Epidemiological survey
Fatalities
Female
Heat-Shock Proteins - chemistry
Hemolysin Proteins
Histocytochemistry - veterinary
immunoassays
immunoglobulin G
Listeria
Listeria monocytogenes
Listeria monocytogenes - genetics
Listeria monocytogenes - isolation & purification
listeriolysin
Listeriolysin O
listeriosis
Listeriosis - blood
Listeriosis - microbiology
Listeriosis - veterinary
Mass Screening - methods
Mass Screening - veterinary
Methods
Microbiology
Milk
Milk - microbiology
Ovine listeriosis
PCR
polymerase chain reaction
Polymerase Chain Reaction - veterinary
rapid methods
Sheep
sheep diseases
Sheep Diseases - blood
Sheep Diseases - microbiology
Veterinary medicine
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Summary:A combined serological and PCR method for the detection of Listeria monocytogenes infection in symptomatic and asymptomatic ovine flocks was evaluated. Seventy-eight milk samples and 157 serum samples were analysed using a L. monocytogenes PCR detection kit and an anti-listeriolysin O IgG immunoassay kit. The combined use of these commercial kits allowed a rapid and effective detection of L. monocytogenes infection in both the early stage, before seroconversion, and in a later phase, even after antibiotic therapy.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2005.12.006