Heme oxygenase-1 mediates the anti-inflammatory effect of mushroom Phellinus linteus in LPS-stimulated RAW264.7 macrophages

• Published in: Journal of ethnopharmacology 2006-07, Vol.106 (3), p.364-371
• Main Authors: Kim, Byung-Chul, Choi, Joung-Woo, Hong, Hye-Young, Lee, Sin-Ae, Hong, Suntaek, Park, Eun-Hee, Kim, Seong-Jin, Lim, Chang-Jin
• Format: Article
• Language: English
• Subjects: Agaricales; Animals; Anti-inflammatory; anti-inflammatory activity; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Biological and medical sciences; c-Jun NH 2-terminal kinase; chemical constituents of plants; enzyme activity; fruiting bodies; Fruiting Bodies, Fungal; General pharmacology; heme oxygenase (decyclizing); Heme oxygenase-1; Heme Oxygenase-1 - antagonists & inhibitors; Heme Oxygenase-1 - biosynthesis; inflammation; Lipopolysaccharides; macrophages; Macrophages - drug effects; Macrophages - metabolism; Medical sciences; medicinal plants; medicinal properties; Mice; mycelium; Nitric oxide; Nitric Oxide - metabolism; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phellinus; Phellinus linteus; Phytotherapy; plant extracts; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Protoporphyrins; Reactive oxygen species
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2006.01.009

This work aimed to elucidate the anti-inflammatory mechanism of the n-BuOH subfraction (PL) prepared from fruiting bodies of Phellinus linteus. PL induced heme oxygenase-1 (HO-1) of the RAW264.7 macrophages in concentration- and time-dependent manner. It suppressed induction of inducible nitric oxide synthase (iNOS) and subsequent production of nitric oxide (NO) through down-regulation of iNOS promoter activity in lipopolysaccharide (LPS)-stimulated macrophages. Zn(II) protoporphyrin IX (ZnPP), a specific inhibitor of HO-1, partly blocked suppression by PL on iNOS promoter activity and NO production, which were elevated in LPS-stimulated macrophages. LPS was able to enhance NO production via reactive oxygen species (ROS) generation, c-Jun NH 2-terminal kinase (JNK) and c-Jun induction. ZnPP prevented PL from down-regulating ROS generation and JNK activation in LPS-stimulated macrophages. Taken together, PL shows its anti-inflammatory activity via mediation of HO-1 in an in vitro inflammation model.