Effect of saccharide length on the immunogenicity of neoglycoconjugates from synthetic fragments of the O-SP of Vibrio cholerae O1, serotype Ogawa

• Published in: Carbohydrate research 2005-10, Vol.340 (14), p.2256-2269
• Main Authors: Saksena, Rina, Ma, Xingquan, Wade, Terri K., Kováč, Pavol, Wade, William F.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - biosynthesis; Antibodies, Bacterial - blood; Cholera vaccine; Conjugate; Dose-Response Relationship, Immunologic; Female; Glycoconjugates - immunology; Immunoglobulin M - biosynthesis; Immunoglobulin M - blood; Lipopolysaccharides - immunology; LPS; Mice; Mice, Inbred BALB C; Neoglycoconjugate; O Antigens - chemistry; O Antigens - immunology; Ogawa; Peptide Fragments - chemistry; Peptide Fragments - immunology; Serotyping; Vibrio cholerae O1; Vibrio cholerae O1 - classification; Vibrio cholerae O1 - immunology
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2005.07.017

The binding energy (90%) for an Ogawa-specific Fab (antigen binding component of the antibody) to bind Ogawa LPS is contributed by the terminal perosamine of the Ogawa LPS. Thus, Ogawa-specific immunogens with equal moles of the terminal perosamine of Ogawa LPS induce equivalent titers of anti-Ogawa antibody independent of the extra mass of additional perosamines. A synthetic hexasaccharide, identical to the terminal hexasaccharide of Ogawa LPS, coupled to bovine serum albumin induced protective antibodies in mice. To determine if there was a minimum saccharide length required for immunogenicity and efficacy, shorter (mono- to pentasaccharide) neoglycoconjugates (CHO–BSA) were tested in mice. The Ogawa CHO–BSA was inoculated at either a constant mass but differing moles, or equal moles but differing masses. Humoral responses were essentially the same when mice received 9 μg of the carbohydrate (0.007 mM with the pentasaccharide) in each of the neoglycoconjugates prepared from mono- through the pentasaccharide, or the same molar amount (0.007 mM), proportionally less by weight when going from the penta- to the monosaccharide. These data show that, within this dose range, the responses occurred virtually independently of the amount of immunogen. Humoral antibodies induced by these immunogens were generally not vibriocidal. Selected antisera induced by CHO–BSA immunogens were protective, but the ELISA titers of the sera were not predictive of the protective capacity. Purified, Ogawa LPS induced anti-Ogawa LPS IgM antibody titers similar to those induced by the Ogawa CHO–BSA conjugates. The anti-whole LPS sera were strongly vibriocidal, as were the previously reported sera induced by hexasaccharide conjugates. This suggests either that the shorter oligosaccharides lack a conformational epitope provided by the hexasaccharide or that the LPS has additional B cell epitopes or selects different B cells in the primary response.