Vaccination of sheep against M. paratuberculosis: immune parameters and protective efficacy

Johne's disease in ruminants is caused by the pathogenic bacterium Mycobacterium avium subspecies paratuberculosis ( Map). Currently available Map commercial vaccines protect against clinical disease but not infection. In this study, the proprietary Johne's vaccine Neoparasec™ and an aqueo...

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Bibliographic Details
Published in:Vaccine 2005-10, Vol.23 (42), p.4999-5008
Main Authors: Begg, D.J., Griffin, J.F.T.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - blood
Applied microbiology
B-Lymphocytes - immunology
Bacterial Vaccines - administration & dosage
Bacterial Vaccines - immunology
Biological and medical sciences
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Interferon-gamma - analysis
Intestinal Mucosa - immunology
Johne's disease
Lymphocyte Activation
Lymphocyte Subsets - immunology
Male
Microbiology
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis - immunology
Paratuberculosis - prevention & control
Protective immunity
Receptors, Interleukin-2 - analysis
Ruminantia
Sheep
Sheep Diseases - prevention & control
Spleen - immunology
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Johne's disease in ruminants is caused by the pathogenic bacterium Mycobacterium avium subspecies paratuberculosis ( Map). Currently available Map commercial vaccines protect against clinical disease but not infection. In this study, the proprietary Johne's vaccine Neoparasec™ and an aqueous formulation of Map 316F ( AquaVax) were tested in sheep. Detailed immunological examination of blood and gut-associated lymphoid tissues was carried out on animals after vaccination and challenge with virulent Map to identify markers of protective immunity. Neoparasec™ vaccination provided significant protection against disease while AquaVax did not. Immune animals had stronger cell-mediated responses and altered proportions of CD4 +, CD8 +, CD25 + and B cells in blood, spleen and the gut lymphatics, than diseased animals.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2005.05.031