A novel neisserial shuttle plasmid: A useful new tool for meningococcal research

We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Ps...

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Bibliographic Details
Published in:FEMS microbiology letters 2005-10, Vol.251 (1), p.143-147
Main Authors: O’ Dwyer, Clíona A., Langford, Paul R., Kroll, J. Simon
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Acinetobacter baumannii - genetics
Amino Acid Sequence
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
E coli
Escherichia coli
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genetic Markers
Genetic Vectors
Genetics
Genetics, Microbial - methods
Kanamycin
Kanamycin Resistance - genetics
Microbiology
Molecular Sequence Data
Neisseria - genetics
Neisseria meningitidis
Neisseria meningitidis - genetics
Nucleotide sequence
Nucleotides
Open Reading Frames
Plasmid
Plasmids - isolation & purification
Proteins
Pseudomonas
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Shuttle vector
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Summary:We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in Escherichia coli and Neisseria meningitidis, and can be used as a shuttle plasmid in meningococcal research.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2005.07.036