Elevated mutation rates in the germline of Polkappa mutant male mice

Mutation rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of DNA polymerase kappa (Polkappa(-/-)) deficient mice. The spontaneous mutation rate in homozygous Polkappa(-/-) males was significantly higher than in isogenic wild-type mice (Polkappa(+/+)), but the ESTR...

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Bibliographic Details
Published in:DNA repair 2006-07, Vol.5 (7), p.860-862
Main Authors: Burr, Karen L-A, Velasco-Miguel, Susana, Duvvuri, Venkata S, McDaniel, Lisa D, Friedberg, Errol C, Dubrova, Yuri E
Format: Article
Language:English
Subjects:
Animals
DNA Damage
DNA Repair - genetics
DNA-Directed DNA Polymerase - deficiency
DNA-Directed DNA Polymerase - genetics
Germ-Line Mutation
Male
Mice
Mice, Inbred BALB C
Mice, Knockout
Minisatellite Repeats
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Summary:Mutation rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of DNA polymerase kappa (Polkappa(-/-)) deficient mice. The spontaneous mutation rate in homozygous Polkappa(-/-) males was significantly higher than in isogenic wild-type mice (Polkappa(+/+)), but the ESTR mutation spectrum in Polkappa(-/-) animals did not differ from that in Polkappa(+/+) males. We suggest that compromised translesion synthesis in Polkappa(-/-) mice may result in replication fork pausing which, in turn, may affect ESTR mutation rate.
ISSN:1568-7864