Activation of telomerase and expression of human telomerase reverse transcriptase in coronary atherosclerosis

Considerable research on telomerase on human neoplastic and normal long-lived proliferative tissues has emerged. We explored the expression of telomerase in atherosclerotic human epicardial coronary arteries. Forty discrete human coronary arterial segments obtained from 19 heart transplant recipient...

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Bibliographic Details
Published in:Cardiovascular pathology 2005-09, Vol.14 (5), p.232-240
Main Authors: Liu, Shih-Chi, Wang, Shoei-Shen, Wu, Mu-Zon, Wu, Deng-Chyang, Yu, Fang-Jung, Chen, Wen-Jone, Chiang, Fu-Tien, Yu, Meng-Fen
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Atherosclerosis
Coronary artery disease
Coronary Artery Disease - enzymology
Coronary Vessels - enzymology
DNA-Binding Proteins - metabolism
Enzyme-Linked Immunosorbent Assay
Gene Amplification
Human telomerase reverse transcriptase (hTERT)
Humans
Immunohistochemistry
Ki-67 antigen
Middle Aged
Polymerase Chain Reaction
Telomerase
Telomerase - metabolism
Telomere
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Summary:Considerable research on telomerase on human neoplastic and normal long-lived proliferative tissues has emerged. We explored the expression of telomerase in atherosclerotic human epicardial coronary arteries. Forty discrete human coronary arterial segments obtained from 19 heart transplant recipients were classified into nonatherosclerotic and atherosclerotic groups based on coronary angiography and histological examination. PCR-ELISA-based telomeric repeat amplification protocol (TRAP), and immunohistochemical analyses were conducted to determine the functional activity and cell-specific expression of telomerase. Seventy percent of atherosclerotic coronary arteries exhibited positive telomerase activity, and the reactivation incidence reached fourfold higher than that of controls ( P=.007). The telomerase catalytic protein, human telomerase reverse transcriptase (hTERT), was expressed in 88% of atherosclerotic tissues, a fivefold higher frequency compared with that of the controls. There was also a correlation of hTERT expression with the level of telomerase bioactivity ( P=.017) and with the severity of atherosclerotic grade ( P
ISSN:1054-8807
1879-1336
DOI:10.1016/j.carpath.2005.05.006