Isolation and RNA1 nucleotide sequence determination of a new insect nodavirus from Pieris rapae larvae in Wuhan city, China

A new insect nodavirus is isolated from Pieris rapae larvae in Wuhan city, China and tentatively designated Wuhan nodavirus (WhNV). We here report the physicochemical characterization of WhNV and determine the nucleotide sequences of its larger segment of genome, RNA1. The results show that WhNV par...

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Bibliographic Details
Published in:Virus research 2006-09, Vol.120 (1), p.28-35
Main Authors: Liu, Chuanfeng, Zhang, Jiamin, Yi, Fuming, Wang, Junping, Wang, Xiaochun, Jiang, Hong, Xu, Jia, Hu, Yuanyang
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Black beetle virus
Boolarra virus
Butterflies - virology
Capsid Proteins - chemistry
Capsid Proteins - isolation & purification
China
Electrophoresis, Gel, Two-Dimensional
Flock house virus
Larva - virology
Molecular Sequence Data
Molecular Weight
Nodamura virus
Nodaviridae - chemistry
Nodaviridae - classification
Nodaviridae - genetics
Nodaviridae - isolation & purification
Nodavirus
Nodaviruses
Pariacoto virus
Phylogeny
Pieris rapae
Protein A
RNA Replicase - genetics
RNA, Viral - genetics
Species Specificity
ssRNA
Viral Proteins - genetics
Wuhan nodavirus
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Summary:A new insect nodavirus is isolated from Pieris rapae larvae in Wuhan city, China and tentatively designated Wuhan nodavirus (WhNV). We here report the physicochemical characterization of WhNV and determine the nucleotide sequences of its larger segment of genome, RNA1. The results show that WhNV particles are isometric, non-enveloped, with a diameter of about 29 nm. The virus has a major capsid protein and a minor capsid protein with estimated molecular mass of 40 and 44 kDa, respectively. WhNV RNA1 is determined to be 3149 nt long, containing a 1014-amino-acid open reading frame (ORF) encoding protein A with a calculated molecular mass of 114,608 Da. The protein A shows 39 and 27% identity to its homologues in Pariacoto virus (PaV) and Striped jack necrosis nervous virus (SJNNV), respectively, but shows only 24% or less identity to its homologues in other insect Nodaviruses such as Nodamura virus (NoV), Black beetle virus (BBV), Boolarra virus (BoV) and Flock house virus (FHV). Predicted domains for six RNA-dependent RNA polymerase motifs and putative ORFs (protein B) are confirmed by sequence analysis of WhNV RNA1.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2005.09.003