Potential anticancer activity of litchi fruit pericarp extract against hepatocellular carcinoma in vitro and in vivo

Litchi fruit pericarp (LFP) extract contains significant amounts of polyphenolic compounds, and exhibits powerful antioxidative activity against fat oxidation in vitro. The purpose of this study is to confirm the anticancer activity of LFP extract against hepatocellular carcinoma in vitro and in viv...

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Bibliographic Details
Published in:Cancer letters 2006-07, Vol.239 (1), p.144-150
Main Authors: Wang, Xiujie, Wei, Yuquan, Yuan, Shulan, Liu, Guanjian, Zhang, Yanrong Lu Jie, Wang, Wendong
Format: Article
Language:English
Subjects:
Animals
Anticancer activity
Antioxidants
Apoptosis
Apoptosis - drug effects
Bromodeoxyuridine - metabolism
Cancer therapies
Carcinoma, Hepatocellular - drug therapy
Cell cycle
Cell growth
Cell Line, Tumor
Cell Proliferation - drug effects
Cytotoxicity
Deoxyribonucleic acid
DNA
Ethanol
Female
Fruits
Growth inhibition
Hepatocellular carcinoma
Humans
In Vitro Techniques
Litchi - chemistry
Litchi fruit pericarp (LFP) extract
Liver cancer
Liver Neoplasms - drug therapy
Mice
Mice, Inbred ICR
Natural products
Phytotherapy
Plant Extracts - therapeutic use
Polyphenols
Survival Rate
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Summary:Litchi fruit pericarp (LFP) extract contains significant amounts of polyphenolic compounds, and exhibits powerful antioxidative activity against fat oxidation in vitro. The purpose of this study is to confirm the anticancer activity of LFP extract against hepatocellular carcinoma in vitro and in vivo, and to elucidate the mechanism of its activity. Human hepatocellular carcinoma cell line was tested in vitro for cytotoxicity, colony formation inhibition, and cell cycle distribution through flow cytometry after treatment with water-soluble crude ethanolic extract (CEE) from LFP. Murine hepatoma bearing-mice were fed doses of 0.15, 0.3, and 0.6 g/kg/day of water-soluble CEE in DH 2O p.o. for 10 days, respectively, to test the anticancer activity and BrdU incorporation of cancer cells in vivo. LFP extract demonstrated a dose- and time-dependent inhibitory effect on cancer cell growth; IC 50 was 80 μg/ml, and significantly inhibited colony formation in vitro, tumor growth and BrdU incorporation into cancer cells in vivo. The tumor inhibitory rates at doses of 0.15, 0.3, and 0.6 g/kg/day were 17.31% ( P>0.05), 30.77% ( P
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2005.08.011