Rapid identification of Candida tropicalis from canine cystitis

The isolate from urine of a dog with cystitis was molecularly identified Candida tropicalis and its minimum inhibitory concentration (MIC) was determined by a microdilution method. The 25S ribosomal DNA sequence analysis indicated that the clinical isolate was essentially identical to that of C. tro...

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Bibliographic Details
Published in:Mycopathologia (1975) 2005-09, Vol.160 (2), p.159-162
Main Authors: Ozawa, H, Okabayashi, K, Kano, R, Watari, T, Watanabe, S, Hasegawa, A
Format: Article
Language:English
Subjects:
Animals
Antifungal Agents - pharmacology
Antifungal Agents - therapeutic use
antifungal properties
Blood
Candida tropicalis
Candida tropicalis - classification
Candida tropicalis - drug effects
Candida tropicalis - genetics
Candida tropicalis - isolation & purification
candidiasis
Candidiasis - microbiology
Candidiasis - veterinary
cystitis
Cystitis - microbiology
Cystitis - veterinary
DNA, Fungal - analysis
DNA, Ribosomal - analysis
dog diseases
Dog Diseases - microbiology
Dogs
drug therapy
fluconazole
Genetic testing
Infections
itraconazole
Itraconazole - pharmacology
Itraconazole - therapeutic use
Male
Microbial Sensitivity Tests
minimum inhibitory concentration
Mycological Typing Techniques
nucleotide sequences
pathogen identification
Ribosomal DNA
RNA, Ribosomal - genetics
Sequence Analysis, DNA
Time Factors
Urine
Urine - microbiology
Veterinary colleges
Veterinary medicine
Yeast
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Description
Summary:The isolate from urine of a dog with cystitis was molecularly identified Candida tropicalis and its minimum inhibitory concentration (MIC) was determined by a microdilution method. The 25S ribosomal DNA sequence analysis indicated that the clinical isolate was essentially identical to that of C. tropicalis and distinct from other Candida species. The MIC(50) and the MIC(90) of fluconazole (FLZ) for the clinical isolate of C. tropicalis was 6.25 and 25 microg/ml, respectively, indicating that susceptibility of the clinical isolate of C. tropicalis to FLZ was less than for other strains of C. tropicalis as well as C. albicans. The molecular analysis as presented in this study assisted the diagnosis of candidiasis by identifying the yeasts in urine samples within 2 days. The patient dog, a 10-year-old male Shih Tzu dog (7.0 kg) referred for examination of cystitis was successfully treated with itraconazole.
ISSN:0301-486X
1573-0832
DOI:10.1007/s11046-005-1151-6