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Metabolism of perfused pig intercostal muscles evaluated by 31P-magnetic resonance spectroscopy
This study presents a perfused preparation for evaluation of metabolism in pig intercostal muscle in vitro . Preserved vessels and nerves to an intercostal segment including two adjacent ribs allowed for tissue perfusion and electrical stimulation with measurement of contraction force, oxygen consum...
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Published in: | Experimental physiology 2006-07, Vol.91 (4), p.755-763 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Request full text |
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Summary: | This study presents a perfused preparation for evaluation of metabolism in pig intercostal muscle in vitro . Preserved vessels and nerves to an intercostal segment including two adjacent ribs allowed for tissue perfusion and electrical
stimulation with measurement of contraction force, oxygen consumption and 31 P-magnetic resonance spectroscopy ( 31 P-MRS). When perfused at rest with KrebsâRinger buffer, the preparation maintained physiological levels of phosphocreatine
(PCr), inorganic phosphate (P i ), ATP and pH at a stable oxygen consumption of 0.51 ± 0.01 μmol min â1 g â1 for more than 2 h. Tonic stimulation of the nerve caused anaerobic energy consumption as PCr and pH decreased, and both variables
recovered after the contraction with half-time values of â¼7 min. Force increased to 0.040 N g â1 (range, 0.031â0.103 N g â1 ) and it gradually decreased by about 70% during the subsequent 5 min of stimulation. The calculated free ADP concentration
increased from 7.4 ± 2.1 nmol g â1 at rest to 28 ± 12 nmol g â1 (mean ±
s.d. ) by the end of the stimulation. Thus anaerobic ATP turnover was zero at rest, 6.1 ± 2 μmol min â1 g â1 during the first minute of stimulation and 3.5 ± 0.5 μmol min â1 g â1 during the two last minutes, corresponding to the drop in force. When the preparation was left unperfused, anaerobic ATP
turnover averaged 0.40 ± 0.15 μmol min â1 g â1 for the first 10 min. The preparation can also be applied to human intercostal muscles, as demonstrated in one preliminary
experiment. The results demonstrate a stable and functional in vitro preparation of intact perfused intercostal muscles in the pig. |
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ISSN: | 0958-0670 1469-445X |
DOI: | 10.1113/expphysiol.2006.033274 |