Developmental expression and hormonal regulation of different isoforms of the transcription factor E75 in the tobacco hornworm Manduca sexta

• Published in: Developmental biology 2006-07, Vol.295 (2), p.623-632
• Main Authors: Keshan, Bela, Hiruma, Kiyoshi, Riddiford, Lynn M.
• Format: Article
• Language: English
• Subjects: Adult differentiation; Animals; Cloning, Molecular; E75; Ecdysone; Ecdysteroid; Ecdysteroids - analysis; Gene Expression Regulation, Developmental; Insect Proteins - genetics; Insect Proteins - physiology; Juvenile hormone; Juvenile Hormones - pharmacology; Larva; Life Cycle Stages - genetics; Manduca; Manduca - genetics; Manduca - physiology; Manduca sexta; Metamorphosis; Molting; Molting - genetics; Protein Isoforms - genetics; Pupal development; RNA, Messenger - analysis; Transcription Factors; Wings, Animal - chemistry
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/j.ydbio.2006.03.049

E75A and E75B, isoforms of the E75 orphan nuclear receptor, are sequentially up-regulated in the abdominal epidermis of the tobacco hornworm Manduca sexta by 20-hydroxyecdysone (20E) during larval and pupal molts, with E75A also increasing at pupal commitment (Zhou et al ., Dev. Biol. 193, 127–138, 1998). We have now cloned E75C and show that little is expressed in the epidermis during larval life with trace amounts seen just before ecdysis. Instead, E75C is found in high amounts during the development of the adult wings as the ecdysteroid titer is rising, and this increase was prevented by juvenile hormone (JH) that prevented adult development. By contrast, E75D is expressed transiently during the larval and pupal molts as the ecdysteroid titer begins to decline and again just before ecdysis, but in the developing adult wings is expressed on the rise of 20E. Removal of the source of JH had little effect on either E75C or E75D mRNA expression during the larval and pupal molts. At the time of pupal commitment, in vitro experiments show that 20E up-regulates E75D and JH prevents this increase. Neither E75A nor E75D mRNA was up-regulated by JH alone. Thus, E75C is primarily involved in adult differentiation whereas E75D has roles both during the molt and pupal commitment.