General Deoxyribozyme-Catalyzed Synthesis of Native 3‘−5‘ RNA Linkages

An elusive goal for nucleic acid enzymology has been deoxyribozymes that ligate RNA rapidly, sequence-generally, with formation of native 3‘−5‘ linkages, and in preparatively useful yield. Using in vitro selection, we have identified Mg2+- and Zn2+-dependent deoxyribozymes that simultaneously fulfil...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2005-09, Vol.127 (38), p.13124-13125
Main Authors: Purtha, Whitney E, Coppins, Rebecca L, Smalley, Mary K, Silverman, Scott K
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Catalysis
DNA, Catalytic - chemistry
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Ligases
RNA - chemical synthesis
Time Factors
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Summary:An elusive goal for nucleic acid enzymology has been deoxyribozymes that ligate RNA rapidly, sequence-generally, with formation of native 3‘−5‘ linkages, and in preparatively useful yield. Using in vitro selection, we have identified Mg2+- and Zn2+-dependent deoxyribozymes that simultaneously fulfill all four of these criteria. The new deoxyribozymes operate under practical incubation conditions and have modest RNA substrate sequence requirements, specifically D↓RA for 9DB1 and A↓R for 7DE5 (D = A, G, or U; R = A or G). These requirements are comparable to those of deoxyribozymes such as 10−23 and 8−17, which are already widely used as biochemical tools for RNA cleavage. We anticipate that the 9DB1 and 7DE5 deoxyribozymes will find immediate practical application for RNA ligation.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0533702