Effects of N,N-bis(3-aminopropyl)dodecylamine on antioxidant enzyme activities, mitochondrial morphology and metabolism in Aspergillus niger

The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSP) as well as of succinate dehydrogenase (SDG), NADH dehydrogenase (NDG) and fumarate hydratase (FHT) were examined in relation to mitochondrial ultrastructure changes in Aspergillus niger exposed to N,N-bis(3...

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Bibliographic Details
Published in:Folia microbiologica 2006, Vol.51 (1), p.38-44
Main Authors: Kuźniak, E, Wyrwicka, A, Gabara, B, Koziróg, A, Skłodowska, M
Format: Article
Language:English
Subjects:
Amines - pharmacology
Antifungal Agents - pharmacology
Aspergillus niger
Aspergillus niger - drug effects
Aspergillus niger - metabolism
Aspergillus niger - ultrastructure
Catalase - metabolism
Enzyme Inhibitors - pharmacology
Fumarate Hydratase - metabolism
Glutathione Peroxidase - metabolism
Hyphae - drug effects
Hyphae - ultrastructure
Microscopy, Electron, Transmission
Mitochondria - drug effects
Mitochondria - metabolism
Mitochondria - ultrastructure
NADH Dehydrogenase - metabolism
Succinate Dehydrogenase - metabolism
Superoxide Dismutase - metabolism
Time Factors
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Summary:The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSP) as well as of succinate dehydrogenase (SDG), NADH dehydrogenase (NDG) and fumarate hydratase (FHT) were examined in relation to mitochondrial ultrastructure changes in Aspergillus niger exposed to N,N-bis(3-aminopropyl)dodecylamine (Apd) that was shown to exhibit fungicidal activity. There was a progressive increase in SOD, CAT and GSP activities 1 and 4 h after 0.05 and 0.1 % Apd application. However, this was followed by a pronounced activity decrease when 0.05 % Apd treatment was prolonged by 1 d. The destructive effect on fungal morphology was observed when this fungicidal agent was applied at the concentration of 0.1 % for 1 d. In the treated hyphae mitochondria degenerated after all organelles. The morphological malformations of mitochondria had an impact on their metabolic state; however, the activities of SDG, NDG and FHT were affected to a different extent. In A. niger the fungicidal effect of Apd could be mediated by oxidative stress impairing the vital mitochondria-related cellular functions.
ISSN:0015-5632
DOI:10.1007/BF02931448