Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium

This paper describes a platform for real-time sensing of dissolved oxygen in a flowing microfluidic environment using an oxygen-sensitive luminescent dye (platinum octaethylporphyrin ketone) integrated into a micro-oxygenator device. Using a phase-based detection method, the luminescent decay lifeti...

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Bibliographic Details
Published in:Lab on a chip 2005-01, Vol.5 (10), p.1059-1066
Main Authors: Vollmer, Adam P, Probstein, Ronald F, Gilbert, Richard, Thorsen, Todd
Format: Article
Language:English
Subjects:
Biological Assay
Biosensing Techniques
Calibration
Capillaries
Dimethylpolysiloxanes - chemistry
Electrochemistry
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Miniaturization
Oxygen - chemistry
Oxygen - metabolism
Silicones - chemistry
Time Factors
Tissue Engineering - methods
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Summary:This paper describes a platform for real-time sensing of dissolved oxygen in a flowing microfluidic environment using an oxygen-sensitive luminescent dye (platinum octaethylporphyrin ketone) integrated into a micro-oxygenator device. Using a phase-based detection method, the luminescent decay lifetime of the dye was consistent with the linear Stern-Volmer relationship using both gaseous and aqueous samples. Maximum sensor resolution varied between 120-780 ppb across a range of dissolved oxygen (DO) concentrations ranging from 0-42.5 ppm. The sensor was subsequently used to determine the convective mass-transfer characteristics of a multi-layer polydimethylsiloxane (PDMS) microfluidic oxygenator. The membrane-based oxygenator showed excellent agreement with an analytical convection model, and the integrated oxygen sensor was accurate across a wide range of tested flow rates (0.05-5 mL min(-1)). The device is unique for its ease of fabrication and highly flexible configuration, as well as the novel incorporation of oxygen delivery and detection in a single micro-device. Potential applications include tissue engineering, cell culturing, and miniaturized bio-assays that require the delivery and/or detection of precise quantities of oxygen within a microfluidic construct.
ISSN:1473-0197
1473-0189
DOI:10.1039/b508097e