Highly efficient vitrification method for cryopreservation of human oocytes

Two experiments were performed to develop a method to cryopreserve MII human oocytes. In the first experiment, three vitrification methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0.25-ml plastic straws foll...

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Bibliographic Details
Published in:Reproductive biomedicine online 2005, Vol.11 (3), p.300-308
Main Authors: Kuwayama, Masashige, Vajta, Gábor, Kato, Osamu, Leibo, Stanley P
Format: Article
Language:English
Subjects:
Animals
birth
Blastocyst - cytology
Blastocyst - physiology
bovine
Cattle
Cryopreservation - instrumentation
Cryopreservation - methods
Cryotop
Embryo Transfer
Ethylene Glycol - pharmacology
Female
human
Humans
In Situ Hybridization, Fluorescence
Male
Oocytes - drug effects
Oocytes - physiology
Pregnancy
Pregnancy Outcome
Pregnancy Rate
Sperm Injections, Intracytoplasmic
vitrification
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Summary:Two experiments were performed to develop a method to cryopreserve MII human oocytes. In the first experiment, three vitrification methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0.25-ml plastic straws followed by in-straw dilution after warming (ISD method); (ii) vitrification in open-pulled straws (OPS method); and (iii) vitrification in
ISSN:1472-6483
1472-6491
DOI:10.1016/S1472-6483(10)60837-1