Regulated expression of TAF1 in 1-cell mouse embryos

TATA binding protein (TBP) associated factor 1 (TAF1) is a member of the general transcription machinery. Interference in the function of TAF1 causes a broad transcriptional defect in early development. To explore possible roles of TAF1 in embryonic transcriptional silence and zygotic genome activat...

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Bibliographic Details
Published in:Zygote (Cambridge) 2006-08, Vol.14 (3), p.209-215
Main Authors: Wang, Kai, Sun, Feng, Sheng, Hui Z.
Format: Article
Language:English
Subjects:
Animals
Female
Gene Expression Regulation, Developmental - physiology
Histone Acetyltransferases
Male
Mice
TAF1
TATA-Binding Protein Associated Factors - biosynthesis
TATA-Binding Protein Associated Factors - genetics
TBP
Transcription Factor TFIID - biosynthesis
Transcription Factor TFIID - genetics
ZGA
Zygote - metabolism
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Summary:TATA binding protein (TBP) associated factor 1 (TAF1) is a member of the general transcription machinery. Interference in the function of TAF1 causes a broad transcriptional defect in early development. To explore possible roles of TAF1 in embryonic transcriptional silence and zygotic genome activation, we examined the expression of TAF1 in 1-cell mouse embryos. Using an immunofluorescence assay, TAF1 was not detected in embryos in the first few hours after fertilization. TAF1 appeared in pronuclei 6 h post-fertilization and reached a relatively high level before zygotic genome activation. These data show that besides TBP, another critical member of the general transcription machinery such as TAF1 is also absent or at an extremely low level at the outset of development. Combined deficiency in critical members of the general transcription machinery may account for embryonic transcriptional silence.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199406003704