In Vitro-Differentiated Embryonic Stem Cells Give Rise to Male Gametes that Can Generate Offspring Mice

Male gametes originate from a small population of spermatogonial stem cells (SSCs). These cells are believed to divide infinitely and to support spermatogenesis throughout life in the male. Here, we developed a strategy for the establishment of SSC lines from embryonic stem (ES) cells. These cells a...

Full description

Saved in:
Bibliographic Details
Published in:Developmental cell 2006-07, Vol.11 (1), p.125-132
Main Authors: Nayernia, Karim, Nolte, Jessica, Michelmann, Hans W., Lee, Jae Ho, Rathsack, Kristina, Drusenheimer, Nadja, Dev, Arvind, Wulf, Gerald, Ehrmann, Ingrid E., Elliott, David J., Okpanyi, Vera, Zechner, Ulrich, Haaf, Thomas, Meinhardt, Andreas, Engel, Wolfgang
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell Line
Cell physiology
DEVBIO
DNA, Recombinant - genetics
Embryo Transfer
Female
Fundamental and applied biological sciences. Psychology
Green Fluorescent Proteins - genetics
In Vitro Techniques
Luminescent Proteins - genetics
Male
Meiosis
Mice
Mice, Transgenic
Molecular and cellular biology
Pregnancy
Recombinant Proteins - genetics
Red Fluorescent Protein
Sperm Injections, Intracytoplasmic
Spermatogenesis
Spermatogonia - cytology
Spermatogonia - metabolism
Stem Cell Transplantation
Stem Cells - cytology
Stem Cells - metabolism
STEMCELL
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Male gametes originate from a small population of spermatogonial stem cells (SSCs). These cells are believed to divide infinitely and to support spermatogenesis throughout life in the male. Here, we developed a strategy for the establishment of SSC lines from embryonic stem (ES) cells. These cells are able to undergo meiosis, are able to generate haploid male gametes in vitro, and are functional, as shown by fertilization after intracytoplasmic injection into mouse oocytes. Resulting two-cell embryos were transferred into oviducts, and live mice were born. Six of seven animals developed to adult mice. This is a clear indication that male gametes derived in vitro from ES cells by this strategy are able to induce normal fertilization and development. Our approach provides an accessible in vitro model system for studies of mammalian gametogenesis, as well as for the development of new strategies for the generation of transgenic mice and treatment of infertility.
ISSN:1534-5807
1878-1551
DOI:10.1016/j.devcel.2006.05.010