Comparison of histometric data obtained by optical coherence tomography and routine histology

There is a lack of systematic investigations comparing optical coherence tomography (OCT) with histology. OCT assessments were performed on the upper back of 16 healthy subjects. Epidermis thickness (ET) was assessed using three methods: first, peak-to-valley analysis of the A-scan (ET-OCT-V); secon...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2005-07, Vol.10 (4), p.044008-044006
Main Authors: Gambichler, Thilo, Boms, Stefanie, Stu¨cker, Markus, Kreuter, Alexander, Sand, Michael, Moussa, Georg, Altmeyer, Peter, Hoffmann, Klaus
Format: Article
Language:English
Subjects:
bioengineering
Biopsy - methods
epidermal thickness
Epidermis - cytology
Histocytological Preparation Techniques - methods
histopathology
Humans
Image Interpretation, Computer-Assisted - methods
interferometry
Microscopy - methods
Middle Aged
noninvasive skin imaging
Reproducibility of Results
Sensitivity and Specificity
Statistics as Topic
Tomography, Optical Coherence - methods
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Summary:There is a lack of systematic investigations comparing optical coherence tomography (OCT) with histology. OCT assessments were performed on the upper back of 16 healthy subjects. Epidermis thickness (ET) was assessed using three methods: first, peak-to-valley analysis of the A-scan (ET-OCT-V); second, manual measurements in the OCT images (ET-OCT-M); third, light microscopic determination using routine histology (ET-Histo). The relationship between the different methods was assessed by means of the Pearson correlation procedure and Bland and Altman plots. We observed a strong correlation between ET-Histo and ET-OCT-V ( , ) and ET-OCT-M ( , ), respectively. Bland and Altman plots revealed a bias of (95 limits of agreement: ) for ET-OCT-V versus ET-Histo and a bias of (95 limits of agreement: ) for ET-OCT-M versus ET-Histo. Despite the strong correlation and low bias observed, the 95 limits of agreement demonstrated an unsatisfactory numerical agreement between the two OCT methods and routine histology indicating that these methods cannot be employed interchangeably. Regarding practicability, precision, and indication spectrum, ET-OCT-V and ET-OCT-M are of different clinical value.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.2039086